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Pillai VV, Siqueira LG, Das M, et al. Physiological profile of undifferentiated bovine blastocyst-derived trophoblasts. Biol Open. 2019;8(5)doi: 10.1242/bio.037937.
Pillai, V. V., Siqueira, L. G., Das, M., Kei, T. G., Tu, L. N., Herren, A. W., Phinney, B. S., Cheong, S. H., Hansen, P. J., & Selvaraj, V. (2019). Physiological profile of undifferentiated bovine blastocyst-derived trophoblasts. Biology open, 8(5), . https://doi.org/10.1242/bio.037937
Pillai, Viju Vijayan, et al. "Physiological profile of undifferentiated bovine blastocyst-derived trophoblasts." Biology open vol. 8,5 (2019). doi: https://doi.org/10.1242/bio.037937
Pillai VV, Siqueira LG, Das M, Kei TG, Tu LN, Herren AW, Phinney BS, Cheong SH, Hansen PJ, Selvaraj V. Physiological profile of undifferentiated bovine blastocyst-derived trophoblasts. Biol Open. 2019 May 01;8(5). doi: 10.1242/bio.037937. PMID: 30952696; PMCID: PMC6550082.
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Biol Open. 2019 May 01;8(5). doi: 10.1242/bio.037937.

Physiological profile of undifferentiated bovine blastocyst-derived trophoblasts.

Biology open

Viju Vijayan Pillai, Luiz G Siqueira, Moubani Das, Tiffany G Kei, Lan N Tu, Anthony W Herren, Brett S Phinney, Soon Hon Cheong, Peter J Hansen, Vimal Selvaraj

Affiliations

  1. Department of Animal Science, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14853, USA.
  2. Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA.
  3. Brazilian Agricultural Research Corporation - Embrapa Gado de Leite, Juiz de Fora, Minas Gerais 36038-330, Brazil.
  4. Genome Center, Proteomics Core Facility, University of California, Davis, CA 95616, USA.
  5. Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
  6. Department of Animal Science, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14853, USA [email protected].

PMID: 30952696 PMCID: PMC6550082 DOI: 10.1242/bio.037937

Abstract

Trophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understanding early events in bovine pregnancy towards initiation of implantation.

© 2019. Published by The Company of Biologists Ltd.

Keywords: Blastocyst; Implantation; Placenta; Pregnancy; Stem cells; Trophoblast

Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

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