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J Food Prot. 1982 Feb;45(2):119-124. doi: 10.4315/0362-028X-45.2.119.

Ultrastructures of Bacteriophages Active Against Streptococcus thermophilus , Lactobacillus bulgaricus , Lactobacillus lactis and Lactobacillus helveticus .

Journal of food protection

G W Reinbold, M S Reddy, E G Hammond

Affiliations

  1. Department of Food Technology, Iowa State University, Ames, Iowa 50011.

PMID: 30866190 DOI: 10.4315/0362-028X-45.2.119

Abstract

Several strains of phages active against Streptococcus thermophilus and species of Lactobacillus were examined with an electron microscope after negative staining with phospho-tungstic acid or uranyl acetate. S. thermophilus bacteriophage exhibited exceptionally long tails (polytails). The width and structure of the polytail was the same as a normal phage tail, 10 nm, but was 2 to 4 times longer, 480-960 nm. Preparations revealed extensive adsorption of S. thermophilus bacteriophage to broken bacterial cell walls. One strain of S. thermophilus phage had a spherical structure at the posterior end of its tail. The bacteriophages of Lactobacillus bulgaricus and Lactobacillus helveticus had a distinct contractile tail sheath, whereas Lactobacillus lactis phage did not.

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