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Schiller HB, Montoro DT, Simon LM, et al. The Human Lung Cell Atlas: A High-Resolution Reference Map of the Human Lung in Health and Disease. Am J Respir Cell Mol Biol. 2019;61(1):31-41doi: 10.1165/rcmb.2018-0416TR.
Schiller, H. B., Montoro, D. T., Simon, L. M., Rawlins, E. L., Meyer, K. B., Strunz, M., Vieira Braga, F. A., Timens, W., Koppelman, G. H., Budinger, G. R. S., Burgess, J. K., Waghray, A., van den Berge, M., Theis, F. J., Regev, A., Kaminski, N., Rajagopal, J., Teichmann, S. A., Misharin, A. V., & Nawijn, M. C. (2019). The Human Lung Cell Atlas: A High-Resolution Reference Map of the Human Lung in Health and Disease. American journal of respiratory cell and molecular biology, 61(1), 31-41. https://doi.org/10.1165/rcmb.2018-0416TR
Schiller, Herbert B, et al. "The Human Lung Cell Atlas: A High-Resolution Reference Map of the Human Lung in Health and Disease." American journal of respiratory cell and molecular biology vol. 61,1 (2019): 31-41. doi: https://doi.org/10.1165/rcmb.2018-0416TR
Schiller HB, Montoro DT, Simon LM, Rawlins EL, Meyer KB, Strunz M, Vieira Braga FA, Timens W, Koppelman GH, Budinger GRS, Burgess JK, Waghray A, van den Berge M, Theis FJ, Regev A, Kaminski N, Rajagopal J, Teichmann SA, Misharin AV, Nawijn MC. The Human Lung Cell Atlas: A High-Resolution Reference Map of the Human Lung in Health and Disease. Am J Respir Cell Mol Biol. 2019 Jul;61(1):31-41. doi: 10.1165/rcmb.2018-0416TR. PMID: 30995076; PMCID: PMC6604220.
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Am J Respir Cell Mol Biol. 2019 Jul;61(1):31-41. doi: 10.1165/rcmb.2018-0416TR.

The Human Lung Cell Atlas: A High-Resolution Reference Map of the Human Lung in Health and Disease.

American journal of respiratory cell and molecular biology

Herbert B Schiller, Daniel T Montoro, Lukas M Simon, Emma L Rawlins, Kerstin B Meyer, Maximilian Strunz, Felipe A Vieira Braga, Wim Timens, Gerard H Koppelman, G R Scott Budinger, Janette K Burgess, Avinash Waghray, Maarten van den Berge, Fabian J Theis, Aviv Regev, Naftali Kaminski, Jayaraj Rajagopal, Sarah A Teichmann, Alexander V Misharin, Martijn C Nawijn

Affiliations

  1. 1 Helmholtz Zentrum München, Institute of Lung Biology and Disease, Group Systems Medicine of Chronic Lung Disease, Member of the German Center for Lung Research (DZL), Munich, Germany.
  2. 2 Harvard Stem Cell Institute, Cambridge, Massachusetts.
  3. 3 Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts.
  4. 4 Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Computational Biology, Neuherberg, Germany.
  5. 5 Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, United Kingdom.
  6. 6 Wellcome Sanger Institute, Hinxton, Cambridge, United Kingdom.
  7. 7 Department of Pathology and Medical Biology.
  8. 8 Groningen Research Institute for Asthma and COPD at the University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
  9. 9 Department of Pediatric Pulmonology and Pediatric Allergology, Beatrix Children's Hospital, and.
  10. 10 Division of Pulmonary and Critical Care Medicine, Northwestern University, Chicago, Illinois.
  11. 11 Department of Pulmonology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
  12. 12 Department of Mathematics, Technische Universität München, Munich, Germany.
  13. 13 Klarman Cell Observatory, Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, Massachusetts.
  14. 14 Department of Biology, Howard Hughes Medical Institute and Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts; and.
  15. 15 Pulmonary, Critical Care and Sleep Medicine, Yale School of Medicine, New Haven, Connecticut.

PMID: 30995076 PMCID: PMC6604220 DOI: 10.1165/rcmb.2018-0416TR

Abstract

Lung disease accounts for every sixth death globally. Profiling the molecular state of all lung cell types in health and disease is currently revolutionizing the identification of disease mechanisms and will aid the design of novel diagnostic and personalized therapeutic regimens. Recent progress in high-throughput techniques for single-cell genomic and transcriptomic analyses has opened up new possibilities to study individual cells within a tissue, classify these into cell types, and characterize variations in their molecular profiles as a function of genetics, environment, cell-cell interactions, developmental processes, aging, or disease. Integration of these cell state definitions with spatial information allows the in-depth molecular description of cellular neighborhoods and tissue microenvironments, including the tissue resident structural and immune cells, the tissue matrix, and the microbiome. The Human Cell Atlas consortium aims to characterize all cells in the healthy human body and has prioritized lung tissue as one of the flagship projects. Here, we present the rationale, the approach, and the expected impact of a Human Lung Cell Atlas.

Keywords: Human Cell Atlas; single-cell RNA sequencing; spatial transcriptomics; systems biology

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