Display options
Cite
Belgacem I, Pangallo S, Abdelfattah A, et al. Transcriptomic Analysis of Orange Fruit Treated with Pomegranate Peel Extract (PGE). Plants (Basel). 2019;8(4)doi: 10.3390/plants8040101.
Belgacem, I., Pangallo, S., Abdelfattah, A., Romeo, F. V., Cacciola, S. O., Li Destri Nicosia, M. G., Ballistreri, G., & Schena, L. (2019). Transcriptomic Analysis of Orange Fruit Treated with Pomegranate Peel Extract (PGE). Plants (Basel, Switzerland), 8(4), . https://doi.org/10.3390/plants8040101
Belgacem, Imen, et al. "Transcriptomic Analysis of Orange Fruit Treated with Pomegranate Peel Extract (PGE)." Plants (Basel, Switzerland) vol. 8,4 (2019). doi: https://doi.org/10.3390/plants8040101
Belgacem I, Pangallo S, Abdelfattah A, Romeo FV, Cacciola SO, Li Destri Nicosia MG, Ballistreri G, Schena L. Transcriptomic Analysis of Orange Fruit Treated with Pomegranate Peel Extract (PGE). Plants (Basel). 2019 Apr 17;8(4). doi: 10.3390/plants8040101. PMID: 30999604; PMCID: PMC6524005.
Copy Download .nbib Format: NLM
Share it on

Plants (Basel). 2019 Apr 17;8(4). doi: 10.3390/plants8040101.

Transcriptomic Analysis of Orange Fruit Treated with Pomegranate Peel Extract (PGE).

Plants (Basel, Switzerland)

Imen Belgacem, Sonia Pangallo, Ahmed Abdelfattah, Flora V Romeo, Santa O Cacciola, Maria G Li Destri Nicosia, Gabriele Ballistreri, Leonardo Schena

Affiliations

  1. Dipartimento di Agraria, Università Mediterranea di Reggio Calabria, Località Feo di Vito, 89122 Reggio Calabria, Italy. [email protected].
  2. Dipartimento di Agraria, Università Mediterranea di Reggio Calabria, Località Feo di Vito, 89122 Reggio Calabria, Italy. [email protected].
  3. Dipartimento di Agraria, Università Mediterranea di Reggio Calabria, Località Feo di Vito, 89122 Reggio Calabria, Italy. [email protected].
  4. Department of Ecology, Environment and Plant Sciences, Stockholm University, 106 91 Stockholm, Sweden. [email protected].
  5. Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria (CREA) ? Centro di Ricerca Olivicoltura, Frutticoltura e Agrumicoltura, Corso Savoia 190, 95024 Acireale, Italy. [email protected].
  6. Dipartimento di Agricoltura, Alimentazione e Ambiente, Università degli Studi di Catania, Via S. Sofia 100, 95123 Catania, Italy. [email protected].
  7. Dipartimento di Agraria, Università Mediterranea di Reggio Calabria, Località Feo di Vito, 89122 Reggio Calabria, Italy. [email protected].
  8. Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria (CREA) ? Centro di Ricerca Olivicoltura, Frutticoltura e Agrumicoltura, Corso Savoia 190, 95024 Acireale, Italy. [email protected].
  9. Dipartimento di Agraria, Università Mediterranea di Reggio Calabria, Località Feo di Vito, 89122 Reggio Calabria, Italy. [email protected].

PMID: 30999604 PMCID: PMC6524005 DOI: 10.3390/plants8040101

Abstract

A Pomegranate Peel Extract (PGE) has been proposed as a natural antifungal substance with a wide range of activity against plant diseases. Previous studies showed that the extract has a direct antimicrobial activity and can elicit resistance responses in plant host tissues. In the present study, the transcriptomic response of orange fruit toward PGE treatments was evaluated. RNA-seq analyses, conducted on wounded fruits 0, 6, and 24 h after PGE applications, showed a significantly different transcriptome in treated oranges as compared to control samples. The majority (273) of the deferentially expressed genes (DEGs) were highly up-regulated compared to only 8 genes that were down-regulated. Gene Ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis showed the involvement of 1233 gene ontology (GO) terms and 35 KEGG metabolic pathways. Among these, important defense pathways were induced and antibiotic biosynthesis was the most enriched one. These findings may explain the underlying preventive and curative activity of PGE against plant diseases.

Keywords: PGE; RNA-seq; orange; plant defense; pomegranate peel extract; transcriptomics

References

  1. Trends Biochem Sci. 1992 Feb;17(2):82-4 - PubMed
  2. J Exp Bot. 2005 Jun;56(416):1525-33 - PubMed
  3. Bioinformatics. 2005 Sep 15;21(18):3674-6 - PubMed
  4. J Exp Bot. 2006;57(3):449-59 - PubMed
  5. Bioinformatics. 2009 May 1;25(9):1105-11 - PubMed
  6. Mol Plant Microbe Interact. 2009 May;22(5):487-97 - PubMed
  7. Int J Food Microbiol. 2009 Sep 15;134(3):244-8 - PubMed
  8. Biochem Biophys Res Commun. 2010 May 28;396(2):522-7 - PubMed
  9. Nat Biotechnol. 2010 May;28(5):511-5 - PubMed
  10. Mol Plant Pathol. 2002 Sep 1;3(5):371-90 - PubMed
  11. Mutat Res. 2010 Dec 21;703(2):99-107 - PubMed
  12. Genome Biol. 2010;11(10):R106 - PubMed
  13. Plant Signal Behav. 2011 Apr;6(4):607-9 - PubMed
  14. Food Chem. 2011 Jul 15;127(2):807-21 - PubMed
  15. Nat Genet. 2013 Jan;45(1):59-66 - PubMed
  16. Plant Physiol. 2014 Jan;164(1):321-39 - PubMed
  17. Front Plant Sci. 2014 Feb 10;5:17 - PubMed
  18. Bioinformatics. 2014 Aug 1;30(15):2114-20 - PubMed
  19. Molecules. 2015 Jun 30;20(7):11941-58 - PubMed
  20. Biotechnol Adv. 2016 Sep-Oct;34(5):532-549 - PubMed
  21. J Food Sci Technol. 2016 May;53(5):2497-503 - PubMed
  22. Int J Biol Macromol. 2017 Jun;99:499-505 - PubMed
  23. Phytopathology. 2017 Dec;107(12):1462-1467 - PubMed
  24. Biomed Res Int. 2017;2017:9584932 - PubMed
  25. Genes (Basel). 2018 Jun 20;9(6):null - PubMed
  26. Genomics Proteomics Bioinformatics. 2018 Aug;16(4):283-293 - PubMed

Publication Types