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Polymers (Basel). 2016 Aug 03;8(8). doi: 10.3390/polym8080279.

Rheological Properties of DNA Molecules in Solution: Molecular Weight and Entanglement Influences.

Polymers

Lourdes Mónica Bravo-Anaya, Frédéric Pignon, Félix Armando Soltero Martínez, Marguerite Rinaudo

Affiliations

  1. Laboratoire Rhéologie et Procédés (LRP), University Grenoble Alpes, Grenoble F-38000, France. [email protected].
  2. Centre National de la Recherche Scientifique (CNRS), Laboratoire Rhéologie et Procédés (LRP), Grenoble F-38000, France. [email protected].
  3. Departamento de Ingeniería Química, Universidad de Guadalajara, Blvd. M. García Barragán, Guadalajara C.P. 44430, Mexico. [email protected].
  4. Laboratoire Rhéologie et Procédés (LRP), University Grenoble Alpes, Grenoble F-38000, France. [email protected].
  5. Centre National de la Recherche Scientifique (CNRS), Laboratoire Rhéologie et Procédés (LRP), Grenoble F-38000, France. [email protected].
  6. Departamento de Ingeniería Química, Universidad de Guadalajara, Blvd. M. García Barragán, Guadalajara C.P. 44430, Mexico. [email protected].
  7. Biomaterials applications, 6 rue Lesdiguières, Grenoble F-38000, France. [email protected].

PMID: 30974556 PMCID: PMC6432494 DOI: 10.3390/polym8080279

Abstract

Molecular weight, stiffness, temperature, and polymer and ionic concentrations are known to widely influence the viscosity of polymer solutions. Additionally, polymer molecular weight-which is related to its dimensions in solution-is one of its most important characteristics. In this communication, low molecular weight DNA from salmon sperm was purified and then studied in solutions in a wide concentration range (between 0.5 and 1600 mg/mL). The intrinsic viscosity of this low molecular weight DNA sample was firstly determined and the evidence of the overlap concentration was detected around the concentration of 125 mg/mL. The chain characteristics of these short molecules were studied in terms of the influence of their molecular weight on the solution viscosities and on the overlap parameter

Keywords: hydrodynamic behavior; low and high molecular weight DNA; overlap parameter CDNA[η]

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