Display options
Cite
Légiot A, Céré C, Dupoiron T, et al. Mitochondria-Associated Membranes (MAMs) are involved in Bax mitochondrial localization and cytochrome c release. Microb Cell. 2019;6(5):257-266doi: 10.15698/mic2019.05.678.
Légiot, A., Céré, C., Dupoiron, T., Kaabouni, M., Camougrand, N., & Manon, S. (2019). Mitochondria-Associated Membranes (MAMs) are involved in Bax mitochondrial localization and cytochrome c release. Microbial cell (Graz, Austria), 6(5), 257-266. https://doi.org/10.15698/mic2019.05.678
Légiot, Alexandre, et al. "Mitochondria-Associated Membranes (MAMs) are involved in Bax mitochondrial localization and cytochrome c release." Microbial cell (Graz, Austria) vol. 6,5 (2019): 257-266. doi: https://doi.org/10.15698/mic2019.05.678
Légiot A, Céré C, Dupoiron T, Kaabouni M, Camougrand N, Manon S. Mitochondria-Associated Membranes (MAMs) are involved in Bax mitochondrial localization and cytochrome c release. Microb Cell. 2019 Mar 15;6(5):257-266. doi: 10.15698/mic2019.05.678. PMID: 31114795; PMCID: PMC6506693.
Copy Download .nbib Format: NLM
Share it on

Microb Cell. 2019 Mar 15;6(5):257-266. doi: 10.15698/mic2019.05.678.

Mitochondria-Associated Membranes (MAMs) are involved in Bax mitochondrial localization and cytochrome c release.

Microbial cell (Graz, Austria)

Alexandre Légiot, Claire Céré, Thibaud Dupoiron, Mohamed Kaabouni, Nadine Camougrand, Stéphen Manon

Affiliations

  1. Institut de Biochimie et de Génétique Cellulaires, UMR 5095 CNRS & Université de Bordeaux, Campus Carreire, CS61390, 1 Rue Camille Saint-Saëns, 33077 Bordeaux, France.

PMID: 31114795 PMCID: PMC6506693 DOI: 10.15698/mic2019.05.678

Abstract

The distribution of the pro-apoptotic protein Bax in the outer mi-tochondrial membrane (OMM) is a central point of regulation of apoptosis. It is now widely recognized that parts of the endoplasmic reticulum (ER) are closely associated to the OMM, and are actively involved in different signaling processes. We addressed a possible role of these domains, called Mitochon-dria-Associated Membranes (MAMs) in Bax localization and function, by ex-pressing the human protein in a yeast mutant deleted of MDM34, a ERMES (ER-Mitochondria Encounter Structure) component. By affecting MAMs stabil-ity, the deletion of MDM34 altered Bax mitochondrial localization, and de-creased its capacity to release cytochrome c. Furthermore, the deletion of MDM34 decreased the size of an incompletely released, MAMs-associated pool of cytochrome c.

Keywords: Bax; ERMES; Saccharomyces cerevisiae; apoptosis; cytochrome c; density gradients; mitochondria; mitochondria associated membranes

Conflict of interest statement

Conflict of interest: The authors declare no conflict of interest.

References

  1. Eur J Biochem. 1999 Sep;264(2):545-53 - PubMed
  2. Cell. 2000 Nov 10;103(4):645-54 - PubMed
  3. J Biol Chem. 2001 Apr 13;276(15):11615-23 - PubMed
  4. Cell Calcium. 2002 Nov-Dec;32(5-6):363-77 - PubMed
  5. Biochem J. 2003 May 1;371(Pt 3):1013-9 - PubMed
  6. J Biol Chem. 2004 May 14;279(20):21085-95 - PubMed
  7. Mol Cell Biochem. 2004 Jan-Feb;256-257(1-2):73-81 - PubMed
  8. Dev Cell. 2004 Jul;7(1):61-71 - PubMed
  9. J Biol Chem. 2004 Dec 10;279(50):52566-73 - PubMed
  10. J Biol Chem. 2005 Mar 18;280(11):10587-98 - PubMed
  11. EMBO J. 2007 May 2;26(9):2229-39 - PubMed
  12. Apoptosis. 2007 May;12(5):887-96 - PubMed
  13. J Immunol. 2007 Jun 15;178(12):8107-16 - PubMed
  14. Toxicol Pathol. 2007 Jun;35(4):495-516 - PubMed
  15. J Biol Chem. 2007 Nov 30;282(48):35104-12 - PubMed
  16. Biochim Biophys Acta. 2008 Jul-Aug;1777(7-8):980-4 - PubMed
  17. Science. 2009 Jul 24;325(5939):477-81 - PubMed
  18. Biochim Biophys Acta. 2011 Apr;1813(4):521-31 - PubMed
  19. J Mol Biol. 2011 Nov 4;413(4):743-50 - PubMed
  20. Traffic. 2012 Jun;13(6):880-90 - PubMed
  21. Cell Death Differ. 2013 Feb;20(2):333-42 - PubMed
  22. J Virol. 2013 May;87(10):5657-68 - PubMed
  23. J Cell Sci. 2013 Aug 15;126(Pt 16):3563-74 - PubMed
  24. Biochim Biophys Acta. 2014 Apr 4;1841(4):595-609 - PubMed
  25. Nat Rev Mol Cell Biol. 2014 Jan;15(1):49-63 - PubMed
  26. Curr Opin Cell Biol. 2015 Aug;35:7-12 - PubMed
  27. Int J Biochem Cell Biol. 2015 Jul;64:136-46 - PubMed
  28. FEBS Lett. 2016 Jan;590(1):13-21 - PubMed
  29. Mech Ageing Dev. 2017 Jan;161(Pt B):201-210 - PubMed
  30. Mol Cell. 2016 Aug 4;63(3):485-97 - PubMed
  31. Biochim Biophys Acta Biomembr. 2017 Jun;1859(6):1144-1155 - PubMed
  32. Microb Cell. 2016 Dec 05;3(12):597-605 - PubMed
  33. Autophagy. 2017 Apr 3;13(4):654-669 - PubMed
  34. FEBS J. 2018 Feb;285(3):416-431 - PubMed
  35. Adv Exp Med Biol. 2017;997:13-31 - PubMed
  36. Cell Death Differ. 2018 Jan;25(1):27-36 - PubMed
  37. EMBO Rep. 2018 Sep;19(9): - PubMed
  38. Structure. 2018 Oct 2;26(10):1346-1359.e5 - PubMed
  39. J Biol Chem. 1994 Nov 4;269(44):27494-502 - PubMed
  40. J Cell Biol. 1994 Sep;126(6):1361-73 - PubMed
  41. J Cell Biol. 1994 Sep;126(6):1375-91 - PubMed
  42. Anticancer Res. 1996 May-Jun;16(3B):1333-9 - PubMed
  43. J Cell Biol. 1997 Feb 10;136(3):545-53 - PubMed
  44. J Cell Biol. 1997 Dec 1;139(5):1281-92 - PubMed

Publication Types