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Grant SR, Church MJ, Ferrón S, et al. Elemental Composition, Phosphorous Uptake, and Characteristics of Growth of a SAR11 Strain in Batch and Continuous Culture. mSystems. 2019;4(4)doi: 10.1128/mSystems.00218-18.
Grant, S. R., Church, M. J., Ferrón, S., Laws, E. A., & Rappé, M. S. (2019). Elemental Composition, Phosphorous Uptake, and Characteristics of Growth of a SAR11 Strain in Batch and Continuous Culture. mSystems, 4(4), . https://doi.org/10.1128/mSystems.00218-18
Grant, Scott R, et al. "Elemental Composition, Phosphorous Uptake, and Characteristics of Growth of a SAR11 Strain in Batch and Continuous Culture." mSystems vol. 4,4 (2019). doi: https://doi.org/10.1128/mSystems.00218-18
Grant SR, Church MJ, Ferrón S, Laws EA, Rappé MS. Elemental Composition, Phosphorous Uptake, and Characteristics of Growth of a SAR11 Strain in Batch and Continuous Culture. mSystems. 2019 May 21;4(4). doi: 10.1128/mSystems.00218-18. PMID: 31117027; PMCID: PMC6589437.
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mSystems. 2019 May 21;4(4). doi: 10.1128/mSystems.00218-18.

Elemental Composition, Phosphorous Uptake, and Characteristics of Growth of a SAR11 Strain in Batch and Continuous Culture.

mSystems

Scott R Grant, Matthew J Church, Sara Ferrón, Edward A Laws, Michael S Rappé

Affiliations

  1. Department of Oceanography, School of Ocean and Earth Science and Technology, University of Hawaii at Manoa, Honolulu, Hawai'i, USA.
  2. Flathead Lake Biological Station, University of Montana, Polson, Montana, USA.
  3. Daniel K. Inouye Center for Microbial Oceanography: Research and Education, School of Ocean and Earth Science and Technology, University of Hawaii at Manoa, Honolulu, Hawai'i, USA.
  4. Department of Environmental Sciences, College of the Coast and Environment, Louisiana State University, Baton Rouge, Louisiana, USA.
  5. Hawaii Institute of Marine Biology, School of Ocean and Earth Science and Technology, University of Hawaii at Manoa, Kaneohe, Hawai'i, USA [email protected].

PMID: 31117027 PMCID: PMC6589437 DOI: 10.1128/mSystems.00218-18

Abstract

In this study, a strain of SAR11 subgroup IIIa (termed HIMB114) was grown in seawater-based batch and continuous culture in order to quantify cellular features and metabolism relevant to SAR11 ecology. We report some of the first direct measurements of cellular elemental quotas for nitrogen (N) and phosphorus (P) for SAR11, grown in batch culture: 1.4 ± 0.9 fg N and 0.44 ± 0.01 fg P, respectively, that were consistent with the small size of HIMB114 cells (average volume of 0.09 μm

Copyright © 2019 Grant et al.

Keywords: SAR11; bacterial production; biogeochemistry; marine microbiology; phosphorous; respiration; seawater

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