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Herrero P, Rodríguez MÁ, Ras MR, et al. Metabolomics Analyses to Investigate the Role of Diet and Physical Training. Methods Mol Biol. 2019;1978:403-430doi: 10.1007/978-1-4939-9236-2_25.
Herrero, P., Rodríguez, M. �. �., Ras, M. R., Del Pino, A., Arola, L., & Canela, N. (2019). Metabolomics Analyses to Investigate the Role of Diet and Physical Training. Methods in molecular biology (Clifton, N.J.), 1978403-430. https://doi.org/10.1007/978-1-4939-9236-2_25
Herrero, Pol, et al. "Metabolomics Analyses to Investigate the Role of Diet and Physical Training." Methods in molecular biology (Clifton, N.J.) vol. 1978 (2019): 403-430. doi: https://doi.org/10.1007/978-1-4939-9236-2_25
Herrero P, Rodríguez MÁ, Ras MR, Del Pino A, Arola L, Canela N. Metabolomics Analyses to Investigate the Role of Diet and Physical Training. Methods Mol Biol. 2019;1978:403-430. doi: 10.1007/978-1-4939-9236-2_25. PMID: 31119677.
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Methods Mol Biol. 2019;1978:403-430. doi: 10.1007/978-1-4939-9236-2_25.

Metabolomics Analyses to Investigate the Role of Diet and Physical Training.

Methods in molecular biology (Clifton, N.J.)

Pol Herrero, Miguel Ángel Rodríguez, Maria Rosa Ras, Antoni Del Pino, Lluís Arola, Núria Canela

Affiliations

  1. Technological Joint Unit of Omic Sciences, EURECAT-Technological Center of Catalonia, Universitat Rovira i Virgili, Reus, Spain.
  2. Nutrigenomics Research Group, Biochemistry and Biotechnological Department, Universitat Rovira i Virgili, Tarragona, Spain. [email protected].
  3. Biotechnological Area, EURECAT-Technological Center of Catalonia, Reus, Spain. [email protected].

PMID: 31119677 DOI: 10.1007/978-1-4939-9236-2_25

Abstract

The metabolic and physiologic responses to healthy dietary habits and physical exercise have become an increasingly interesting research area, since equilibrated diet and regular physical activity are commonly recommended for their antioxidant capacity and for the prevention and treatment of several disorders as insulin resistance, dyslipidemia, obesity, and hypertension that may result in cardiovascular disease and type II diabetes.Nutritional and exercise-induced responses and the biological mechanisms that explain these associations have been tackled by several researchers using metabolomic approaches that have emerged as a powerful tool to comprehensively evaluate individual metabolic signatures, analyzing metabolome composition in serum, urine, stool, or tissue samples.The overview of the wide range of metabolites related to dietary and to physical training interventions reported from numerous human or animal studies endorses the complexity for assessing metabolic changes and compound identification, and a combination of targeted and non-targeted global profiling studies is recommended for increasing the understanding of nutrition and exercise metabolic mechanisms.The present protocol attempt to identify variations in human blood circulating metabolites with a multiplatform global analysis based on LC-MS, GC-MS, and NMR, combining targeted and untargeted strategies, to complete the holistic understanding of the serum metabolome.

Keywords: Diet; Gas chromatography; Liquid chromatography; Mass spectrometry; Metabolomics; Nuclear magnetic resonance; Nutrition; Physical training

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