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Ha SD, Ricke SC, Nisbet DJ, et al. Serine Utilization as a Potential Competition Mechanism Between Salmonella and a Chicken Cecal Bacterium. J Food Prot. 1994;57(12):1074-1079doi: 10.4315/0362-028X-57.12.1074.
Ha, S. D., Ricke, S. C., Nisbet, D. J., Corrier, D. E., & Deloach, J. R. (1994). Serine Utilization as a Potential Competition Mechanism Between Salmonella and a Chicken Cecal Bacterium. Journal of food protection, 57(12), 1074-1079. https://doi.org/10.4315/0362-028X-57.12.1074
Ha, S D, et al. "Serine Utilization as a Potential Competition Mechanism Between Salmonella and a Chicken Cecal Bacterium." Journal of food protection vol. 57,12 (1994): 1074-1079. doi: https://doi.org/10.4315/0362-028X-57.12.1074
Ha SD, Ricke SC, Nisbet DJ, Corrier DE, Deloach JR. Serine Utilization as a Potential Competition Mechanism Between Salmonella and a Chicken Cecal Bacterium. J Food Prot. 1994 Dec;57(12):1074-1079. doi: 10.4315/0362-028X-57.12.1074. PMID: 31121649.
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J Food Prot. 1994 Dec;57(12):1074-1079. doi: 10.4315/0362-028X-57.12.1074.

Serine Utilization as a Potential Competition Mechanism Between Salmonella and a Chicken Cecal Bacterium.

Journal of food protection

S D Ha, S C Ricke, D J Nisbet, D E Corrier, J R Deloach

Affiliations

  1. Department of Poultry Science, Texas A&M University, College Station, Texas 77843.
  2. U.S. Department of Agriculture, Agriculture Research Service, Food Animal Protection Research Laboratory, Route 5, Box 810, College Station, Texas 77845.

PMID: 31121649 DOI: 10.4315/0362-028X-57.12.1074

Abstract

Our objective in this study was to use batch culture to estimate growth kinetic parameters for a growth-limiting amino acid, serine, in Salmonella typhimurium and a in chicken cecal bacterium, Escherichia fergusonii , and to test these predictions for competitiveness in a mixed culture of the two organisms. Under anaerobic growth conditions, the two bacteria grew only when serine was provided as the nitrogen source, When serine was used as a carbon source in aerobic media, the maximum growth rates of the two organisms were considerably lower and the affinity constants were higher than when serine was used as a nitrogen source. The maximum growth rates of the two organisms were lower in anaerobic media than in aerobic media, but serine anaerobic affinity constants were lower than those from aerobic media. In aerobic mixed culture, S. typhimurium outgrew E. fergusonii in N-limited minimal media containing 0.1 mM serine but in N-nonlimited minimal media containing 10 mM serine, E. fergusonii outgrew S. typhimurium . In anaerobic mixed culture, E. fergusonii outgrew S. typhimurium in media containing both 0.1 mM and 10 mM serine concentrations. It appears that the cause of inhibition of Salmonella with serine as the variable nutrient differs depending on the oxidation-reduction status.

Keywords: ; affinity constants; amino acids; growth rates

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