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Korsrud GO, Papich MG, Fesser ACE, et al. Laboratory Testing of the Charm Test II Receptor Assays and the Charm Farm Test with Tissues and Fluids from Hogs Fed Sulfamethazine, Chlortetracycline, and Penicillin G. J Food Prot. 1996;59(2):161-166doi: 10.4315/0362-028X-59.2.161.
Korsrud, G. O., Papich, M. G., Fesser, A. C. E., Salisbury, C. D. C., & Macneil, J. D. (1996). Laboratory Testing of the Charm Test II Receptor Assays and the Charm Farm Test with Tissues and Fluids from Hogs Fed Sulfamethazine, Chlortetracycline, and Penicillin G. Journal of food protection, 59(2), 161-166. https://doi.org/10.4315/0362-028X-59.2.161
Korsrud, Gary O, et al. "Laboratory Testing of the Charm Test II Receptor Assays and the Charm Farm Test with Tissues and Fluids from Hogs Fed Sulfamethazine, Chlortetracycline, and Penicillin G." Journal of food protection vol. 59,2 (1996): 161-166. doi: https://doi.org/10.4315/0362-028X-59.2.161
Korsrud GO, Papich MG, Fesser ACE, Salisbury CDC, Macneil JD. Laboratory Testing of the Charm Test II Receptor Assays and the Charm Farm Test with Tissues and Fluids from Hogs Fed Sulfamethazine, Chlortetracycline, and Penicillin G. J Food Prot. 1996 Feb;59(2):161-166. doi: 10.4315/0362-028X-59.2.161. PMID: 31158998.
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J Food Prot. 1996 Feb;59(2):161-166. doi: 10.4315/0362-028X-59.2.161.

Laboratory Testing of the Charm Test II Receptor Assays and the Charm Farm Test with Tissues and Fluids from Hogs Fed Sulfamethazine, Chlortetracycline, and Penicillin G.

Journal of food protection

Gary O Korsrud, Mark G Papich, Adrian C E Fesser, Craig D C Salisbury, James D Macneil

Affiliations

  1. Health of Animals Laboratory, Agriculture Canada, 116 Veterinary Road, Saskatoon, Saskatchewan S7N 5E3, Canada.

PMID: 31158998 DOI: 10.4315/0362-028X-59.2.161

Abstract

The potentials of the Charm Test II receptor assays for the detection of residues of sulfonamides, tetracyclines, and β-lactams and the Charm Farm Test for screening for antimicrobial residues were tested. Market hogs were fed rations containing three times the label level of sulfamethazine, chlortetracycline, and penicillin G for 2 weeks. Groups were killed after 0, 2, 4 and 8 days of withdrawal. Quantitative chemical methods were used to determine residue levels in fluids and tissues. Results were compared with those obtained using the qualitative Charm Test II receptor assays and the Charm Farm Test antimicrobial inhibition assay. For the Charm Test II assays for sulfonamides, tetracyclines and β-lactams, respectively, 1.6, 5, and 6% of the results were false positive and 7, 5, and 0% were false negative, based on the limits of detection for the test kits and the quantitative results. On a similar basis for the Charm Farm Test, 16% of the results were false positive (6 kidney, 4 muscle, and 6 urine samples) and 1% were false negative (sulfamethazine in one urine sample).

Keywords: Charm Farm Test; Charm Test II; antimicrobial; residue testing; swine

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