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Mahtab S, Kar P, Saha S, et al. Central Immune Tolerance of T and B Cells in Patients With Idiopathic Hypoparathyroidism, T1D, and Autoimmune Thyroiditis. J Endocr Soc. 2019;3(6):1175-1184doi: 10.1210/js.2018-00344.
Mahtab, S., Kar, P., Saha, S., Sreenivas, V., Sottini, A., Imberti, L., & Goswami, R. (2019). Central Immune Tolerance of T and B Cells in Patients With Idiopathic Hypoparathyroidism, T1D, and Autoimmune Thyroiditis. Journal of the Endocrine Society, 3(6), 1175-1184. https://doi.org/10.1210/js.2018-00344
Mahtab, Samrina, et al. "Central Immune Tolerance of T and B Cells in Patients With Idiopathic Hypoparathyroidism, T1D, and Autoimmune Thyroiditis." Journal of the Endocrine Society vol. 3,6 (2019): 1175-1184. doi: https://doi.org/10.1210/js.2018-00344
Mahtab S, Kar P, Saha S, Sreenivas V, Sottini A, Imberti L, Goswami R. Central Immune Tolerance of T and B Cells in Patients With Idiopathic Hypoparathyroidism, T1D, and Autoimmune Thyroiditis. J Endocr Soc. 2019 Feb 20;3(6):1175-1184. doi: 10.1210/js.2018-00344. eCollection 2019 Jun 01. PMID: 31139764; PMCID: PMC6532674.
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J Endocr Soc. 2019 Feb 20;3(6):1175-1184. doi: 10.1210/js.2018-00344. eCollection 2019 Jun 01.

Central Immune Tolerance of T and B Cells in Patients With Idiopathic Hypoparathyroidism, T1D, and Autoimmune Thyroiditis.

Journal of the Endocrine Society

Samrina Mahtab, Parmita Kar, Soma Saha, Vishnubhatla Sreenivas, Alessandra Sottini, Luisa Imberti, Ravinder Goswami

Affiliations

  1. Department of Endocrinology and Metabolism, All India Institute of Medical Sciences, New Delhi, India.
  2. Department of Biostatistics, All India Institute of Medical Sciences, New Delhi, India.
  3. Diagnostic Department, Centro di Ricerca Emato-oncologica AIL, ASST Spedali Civili di Brescia, Brescia, Italy.

PMID: 31139764 PMCID: PMC6532674 DOI: 10.1210/js.2018-00344

Abstract

CONTEXT: Pathogenesis of idiopathic hypoparathyroidism (IH) is under investigation. Abnormalities in central immune tolerance have yet not been investigated in this condition. T-cell receptor excision circles (TRECs) and kappa-deleting recombination excision circles (KRECs), formed during receptor gene rearrangements, are tools to assess central T- and B-cell output.

OBJECTIVE: We assessed the number of circulating TRECs and KRECs in patients with IH, autoimmune type 1 diabetes (T1D), and autoimmune thyroiditis (ATs) and healthy controls (HCs).

DESIGN: Comparative case-control at tertiary care center.

SUBJECTS AND METHODS: Absolute and relative TRECs and KRECs were measured in DNA extracted from whole blood of patients with IH (n = 181, 22 of whom were reassessed after a decade of follow-up) and T1D (n = 133), AT (n = 53), and HC (n = 135) using a quantitative real-time PCR/TaqMan

RESULTS: Absolute and relative means of TRECs and KRECs in IH were comparable to HCs, and no differences were found between IH with and without calcium-sensing receptor antibodies or class I

CONCLUSION: Patients with IH showed TRECs and KRECs comparable to HCs, indicating an intact mechanism of T- and B-cell central immune tolerance. Interestingly, absolute TRECs were significantly higher in T1D than HCs, suggesting impaired central immune tolerance in T1D.

Keywords: CaSRAb; T-cell receptor excision circles; autoimmunity; idiopathic hypoparathyroidism; kappa-deleting recombination excision circles

References

  1. J Immunol. 1999 Dec 1;163(11):5883-90 - PubMed
  2. Lancet. 2000 May 27;355(9218):1875-81 - PubMed
  3. Clin Exp Immunol. 2001 Sep;125(3):409-13 - PubMed
  4. J Exp Med. 2002 Mar 18;195(6):789-94 - PubMed
  5. Acta Neurol Scand. 2002 Feb;105(2):128-31 - PubMed
  6. J Endocrinol. 2003 Mar;176(3):305-11 - PubMed
  7. J Clin Endocrinol Metab. 2004 Oct;89(10):4840-5 - PubMed
  8. Int J Immunogenet. 2005 Dec;32(6):393-400 - PubMed
  9. Int J Immunogenet. 2006 Aug;33(4):237-40 - PubMed
  10. J Clin Endocrinol Metab. 2006 Nov;91(11):4256-9 - PubMed
  11. Clin Endocrinol (Oxf). 2006 Sep;65(3):359-63 - PubMed
  12. Clin Exp Immunol. 2008 Sep;153(3):338-50 - PubMed
  13. Calif Med. 1946 Jul;65(1):3-5 - PubMed
  14. Br J Nutr. 2009 Aug;102(3):382-6 - PubMed
  15. Blood. 2009 Oct 29;114(18):3803-12 - PubMed
  16. Rejuvenation Res. 2009 Oct;12(5):311-20 - PubMed
  17. Eur J Endocrinol. 2010 Feb;162(2):407-21 - PubMed
  18. Clin Immunol. 2010 Aug;136(2):217-27 - PubMed
  19. Horm Res Paediatr. 2011;76(5):335-42 - PubMed
  20. J Clin Endocrinol Metab. 2012 Apr;97(4):1219-26 - PubMed
  21. Clin Endocrinol (Oxf). 2012 Aug;77(2):200-6 - PubMed
  22. PLoS One. 2012;7(1):e30494 - PubMed
  23. Front Immunol. 2011 May 04;2:12 - PubMed
  24. J Clin Endocrinol Metab. 2012 Sep;97(9):E1820-4 - PubMed
  25. PLoS One. 2012;7(8):e42412 - PubMed
  26. Eur J Endocrinol. 2013 May 07;168(6):895-903 - PubMed
  27. J Transl Med. 2013 May 09;11:119 - PubMed
  28. J Clin Endocrinol Metab. 2013 Sep;98(9):3884-91 - PubMed
  29. BMC Endocr Disord. 2013 Sep 05;13:34 - PubMed
  30. J Clin Lab Anal. 2014 May;28(3):249-54 - PubMed
  31. Eur J Endocrinol. 2014 Apr 10;170(5):777-83 - PubMed
  32. Clin Endocrinol (Oxf). 2014 Nov;81(5):769-74 - PubMed
  33. Ann Med. 2014 Nov;46(7):555-65 - PubMed
  34. J Vis Exp. 2014 Dec 06;(94):null - PubMed
  35. Clin Endocrinol (Oxf). 2016 Apr;84(4):578-86 - PubMed
  36. J Clin Endocrinol Metab. 2016 Jun;101(6):2300-12 - PubMed
  37. J Clin Endocrinol Metab. 2017 Jan 1;102(1):167-175 - PubMed
  38. J Clin Endocrinol Metab. 2017 Jan 1;102(1):251-258 - PubMed
  39. Front Immunol. 2016 Dec 02;7:546 - PubMed
  40. Pediatr Blood Cancer. 2017 Dec;64(12):null - PubMed
  41. Clin Exp Immunol. 1966 Apr;1(2):119-28 - PubMed
  42. J Clin Invest. 1993 Nov;92(5):2313-22 - PubMed
  43. J Clin Invest. 1996 Feb 15;97(4):910-4 - PubMed

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