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Pochechuev MS, Lanin AA, Kelmanson IV, et al. Stain-free subcellular-resolution astrocyte imaging using third-harmonic generation. Opt Lett. 2019;44(12):3166-3169doi: 10.1364/OL.44.003166.
Pochechuev, M. S., Lanin, A. A., Kelmanson, I. V., Bilan, D. S., Kotova, D. A., Chebotarev, A. S., Tarabykin, V., Fedotov, A. B., Belousov, V. V., & Zheltikov, A. M. (2019). Stain-free subcellular-resolution astrocyte imaging using third-harmonic generation. Optics letters, 44(12), 3166-3169. https://doi.org/10.1364/OL.44.003166
Pochechuev, M S, et al. "Stain-free subcellular-resolution astrocyte imaging using third-harmonic generation." Optics letters vol. 44,12 (2019): 3166-3169. doi: https://doi.org/10.1364/OL.44.003166
Pochechuev MS, Lanin AA, Kelmanson IV, Bilan DS, Kotova DA, Chebotarev AS, Tarabykin V, Fedotov AB, Belousov VV, Zheltikov AM. Stain-free subcellular-resolution astrocyte imaging using third-harmonic generation. Opt Lett. 2019 Jun 15;44(12):3166-3169. doi: 10.1364/OL.44.003166. PMID: 31199407.
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Opt Lett. 2019 Jun 15;44(12):3166-3169. doi: 10.1364/OL.44.003166.

Stain-free subcellular-resolution astrocyte imaging using third-harmonic generation.

Optics letters

M S Pochechuev, A A Lanin, I V Kelmanson, D S Bilan, D A Kotova, A S Chebotarev, V Tarabykin, A B Fedotov, V V Belousov, A M Zheltikov

PMID: 31199407 DOI: 10.1364/OL.44.003166

Abstract

We demonstrate stain-free, high-contrast, subcellular-resolution imaging of astroglial cells using epi-detected third-harmonic generation (THG). The astrocyte-imaging capability of THG is verified by colocalizing THG images with fluorescence images of astrocytes expressing a genetically encodable fluorescent reporter. We show that THG imaging with an optimized point-spread function can reliably detect significant subcellular features of astrocytes, including cell nuclei, as well as the soma shape and boundaries.

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