Display options
Cite
Meena DK, Akunuri S, Meena P, et al. Tissue Transglutaminase Antibody and Its Association with Duodenal Biopsy in Diagnosis of Pediatric Celiac Disease. Pediatr Gastroenterol Hepatol Nutr. 2019;22(4):350-357doi: 10.5223/pghn.2019.22.4.350.
Meena, D. K., Akunuri, S., Meena, P., Bhramer, A., Sharma, S. D., & Gupta, R. (2019). Tissue Transglutaminase Antibody and Its Association with Duodenal Biopsy in Diagnosis of Pediatric Celiac Disease. Pediatric gastroenterology, hepatology & nutrition, 22(4), 350-357. https://doi.org/10.5223/pghn.2019.22.4.350
Meena, Daleep K, et al. "Tissue Transglutaminase Antibody and Its Association with Duodenal Biopsy in Diagnosis of Pediatric Celiac Disease." Pediatric gastroenterology, hepatology & nutrition vol. 22,4 (2019): 350-357. doi: https://doi.org/10.5223/pghn.2019.22.4.350
Meena DK, Akunuri S, Meena P, Bhramer A, Sharma SD, Gupta R. Tissue Transglutaminase Antibody and Its Association with Duodenal Biopsy in Diagnosis of Pediatric Celiac Disease. Pediatr Gastroenterol Hepatol Nutr. 2019 Jul;22(4):350-357. doi: 10.5223/pghn.2019.22.4.350. Epub 2019 Jun 20. PMID: 31338310; PMCID: PMC6629588.
Copy Download .nbib Format: NLM
Share it on

Pediatr Gastroenterol Hepatol Nutr. 2019 Jul;22(4):350-357. doi: 10.5223/pghn.2019.22.4.350. Epub 2019 Jun 20.

Tissue Transglutaminase Antibody and Its Association with Duodenal Biopsy in Diagnosis of Pediatric Celiac Disease.

Pediatric gastroenterology, hepatology & nutrition

Daleep K Meena, Shalini Akunuri, Preetam Meena, Ashok Bhramer, Shiv D Sharma, Rajkumar Gupta

Affiliations

  1. Pediatric Intensive Care Unit, Great Ormond Street Hospital, London, United Kingdom.
  2. Department of Pediatrics, Lady Harding Medical College, New Delhi, India.
  3. Department of Pediatrics, Government BDM Hospital, Jaipur, India.
  4. Department of Pediatrics, SMS Medical College, Jaipur, India.

PMID: 31338310 PMCID: PMC6629588 DOI: 10.5223/pghn.2019.22.4.350

Abstract

PURPOSE: This study aimed to evaluate a possible association between the anti-tissue transglutaminase antibody (anti-tTG) titer and stage of duodenal mucosal damage and assess a possible cut-off value of anti-tTG at which celiac disease (CD) may be diagnosed in children in conjunction with clinical judgment.

METHODS: This observational study was conducted at a gastroenterology clinic in a tertiary hospital from April 2012 to May 2013. Seventy children between 6-months and 18-years-old with suspected CD underwent celiac serology and duodenal biopsy. Statistical analyses were done using SPSS 16. Diagnostic test values were determined for comparing the anti-tTG titer with duodenal biopsy. An analysis of variance and Tukey-Kramer tests were performed for comparing the means between groups. A receiver operating characteristics curve was plotted to determine various cut-off values of anti-tTG.

RESULTS: The mean antibody titer increased with severity of Marsh staging (

CONCLUSION: There is an association between the anti-tTG titer and stage of duodenal mucosal injury in children with CD. An anti-tTG value of 115 AU/mL (6.4 times the upper normal limit) had 76% sensitivity, 100% specificity, with a 100% PPV, and 17% NPV for diagnosing CD (95% CI, 0.75-1). This cut-off may be used in combination with clinical judgment to diagnose CD.

Keywords: Antibodies; Celiac disease; Duodenitis; Transglutaminase

Conflict of interest statement

Conflict of Interest: The authors have no financial conflicts of interest.

References

  1. Eur J Gastroenterol Hepatol. 1999 Oct;11(10):1185-94 - PubMed
  2. Pediatrics. 2005 May;115(5):1341-6 - PubMed
  3. Clin Gastroenterol Hepatol. 2006 Sep;4(9):1112-7 - PubMed
  4. J Clin Gastroenterol. 2008 Mar;42(3):256-60 - PubMed
  5. Clin Vaccine Immunol. 2009 Nov;16(11):1576-82 - PubMed
  6. World J Gastroenterol. 2009 Oct 14;15(38):4775-80 - PubMed
  7. Indian J Pathol Microbiol. 2011 Apr-Jun;54(2):279-83 - PubMed
  8. Dig Liver Dis. 2012 Apr;44(4):280-5 - PubMed
  9. J Pediatr Gastroenterol Nutr. 2012 Jan;54(1):136-60 - PubMed
  10. J Pediatr Gastroenterol Nutr. 2012 Jul;55(1):44-9 - PubMed
  11. Middle East J Dig Dis. 2014 Jul;6(3):131-6 - PubMed
  12. Arch Dis Child. 2017 Oct;102(10):942-946 - PubMed

Publication Types