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Nombela I, Lopez-Lorigados M, Salvador-Mira ME, et al. Integrated Transcriptomic and Proteomic Analysis of Red Blood Cells from Rainbow Trout Challenged with VHSV Point Towards Novel Immunomodulant Targets. Vaccines (Basel). 2019;7(3)doi: 10.3390/vaccines7030063.
Nombela, I., Lopez-Lorigados, M., Salvador-Mira, M. E., Puente-Marin, S., Chico, V., Ciordia, S., Mena, M. C., Mercado, L., Coll, J., Perez, L., & Ortega-Villaizan, M. D. M. (2019). Integrated Transcriptomic and Proteomic Analysis of Red Blood Cells from Rainbow Trout Challenged with VHSV Point Towards Novel Immunomodulant Targets. Vaccines, 7(3), . https://doi.org/10.3390/vaccines7030063
Nombela, Ivan, et al. "Integrated Transcriptomic and Proteomic Analysis of Red Blood Cells from Rainbow Trout Challenged with VHSV Point Towards Novel Immunomodulant Targets." Vaccines vol. 7,3 (2019). doi: https://doi.org/10.3390/vaccines7030063
Nombela I, Lopez-Lorigados M, Salvador-Mira ME, Puente-Marin S, Chico V, Ciordia S, Mena MC, Mercado L, Coll J, Perez L, Ortega-Villaizan MDM. Integrated Transcriptomic and Proteomic Analysis of Red Blood Cells from Rainbow Trout Challenged with VHSV Point Towards Novel Immunomodulant Targets. Vaccines (Basel). 2019 Jul 09;7(3). doi: 10.3390/vaccines7030063. PMID: 31324030; PMCID: PMC6789484.
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Vaccines (Basel). 2019 Jul 09;7(3). doi: 10.3390/vaccines7030063.

Integrated Transcriptomic and Proteomic Analysis of Red Blood Cells from Rainbow Trout Challenged with VHSV Point Towards Novel Immunomodulant Targets.

Vaccines

Ivan Nombela, Marina Lopez-Lorigados, Maria Elizabeth Salvador-Mira, Sara Puente-Marin, Veronica Chico, Sergio Ciordia, Maria Carmen Mena, Luis Mercado, Julio Coll, Luis Perez, Maria Del Mar Ortega-Villaizan

Affiliations

  1. Departamento de Bioquímica y Biología Molecular, Instituto de Biología Molecular y Celular (IBMC) and Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche (IDiBE), Universidad Miguel Hernández (UMH), 03202 Elche, Spain.
  2. Unidad de Proteómica, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Madrid, Spain.
  3. Instituto de Biología, Pontificia Universidad Católica de Valparaíso (PUCV), Valparaíso 2373223, Chile.
  4. Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, Spain.
  5. Departamento de Bioquímica y Biología Molecular, Instituto de Biología Molecular y Celular (IBMC) and Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche (IDiBE), Universidad Miguel Hernández (UMH), 03202 Elche, Spain. [email protected].

PMID: 31324030 PMCID: PMC6789484 DOI: 10.3390/vaccines7030063

Abstract

Teleost red blood cells (RBCs) are nucleated and therefore can propagate cellular responses to exogenous stimuli. RBCs can mount an immune response against a variety of fish viruses, including the viral septicemia hemorrhagic virus (VHSV), which is one of the most prevalent fish viruses resulting in aquaculture losses. In this work, RBCs from blood and head kidney samples of rainbow trout challenged with VHSV were analyzed via transcriptomic and proteomic analyses. We detected an overrepresentation of differentially expressed genes (DEGs) related to the type I interferon response and signaling in RBCs from the head kidney and related to complement activation in RBCs from blood. Antigen processing and presentation of peptide antigen was overrepresented in RBCs from both tissues. DEGs shared by both tissues showed an opposite expression profile. In summary, this work has demonstrated that teleost RBCs can modulate the immune response during an in vivo viral infection, thus implicating RBCs as cell targets for the development of novel immunomodulants.

Keywords: IFIT5; Mx; VHSV; antigen presentation; complement; erythrocytes; interferon; proteome; red blood cells; rhabdoviruses; transcriptome; β-defensin 1

References

  1. J Virol. 1997 Jul;71(7):5304-11 - PubMed
  2. J Leukoc Biol. 2016 Jul;100(1):27-45 - PubMed
  3. J Mol Biol. 2014 Mar 20;426(6):1210-9 - PubMed
  4. Front Immunol. 2019 Mar 04;10:160 - PubMed
  5. J Immunol Res. 2018 Apr 30;2018:9480497 - PubMed
  6. Fish Shellfish Immunol. 2013 Feb;34(2):632-40 - PubMed
  7. Fish Shellfish Immunol. 2016 Mar;50:1-10 - PubMed
  8. Vaccine. 2011 Jan 17;29(4):737-43 - PubMed
  9. Acta Biochim Biophys Sin (Shanghai). 2013 Oct;45(10):867-74 - PubMed
  10. Genes (Basel). 2018 Apr 09;9(4):null - PubMed
  11. Nature. 2007 Apr 19;446(7138):916-920 - PubMed
  12. Bioinformatics. 2009 Apr 15;25(8):1091-3 - PubMed
  13. Nature. 2008 Jan 31;451(7178):573-7 - PubMed
  14. F1000Res. 2017 Nov 06;6:1958 - PubMed
  15. Biochimie. 2007 Jun-Jul;89(6-7):812-8 - PubMed
  16. Protein Sci. 2001 Dec;10(12):2470-9 - PubMed
  17. Proc Natl Acad Sci U S A. 2007 Jan 9;104(2):582-7 - PubMed
  18. Nucleic Acids Res. 2008 Jun;36(10):3420-35 - PubMed
  19. PLoS Pathog. 2018 Apr 26;14(4):e1006910 - PubMed
  20. Front Immunol. 2019 Apr 16;10:613 - PubMed
  21. Microbiol Mol Biol Rev. 2013 Dec;77(4):551-66 - PubMed
  22. Front Immunol. 2018 Oct 29;9:2477 - PubMed
  23. J Virol. 2015 Jul;89(14):6974-7 - PubMed
  24. Cell Death Dis. 2019 Apr 25;10(5):346 - PubMed
  25. Bioinformatics. 2013 Mar 1;29(5):661-3 - PubMed
  26. Nature. 2018 Jun;558(7711):610-614 - PubMed
  27. Immunology. 2004 Mar;111(3):273-81 - PubMed
  28. J Virol Methods. 2006 Mar;132(1-2):154-9 - PubMed
  29. Nat Genet. 2014 May;46(5):503-509 - PubMed
  30. Virology. 2006 Aug 15;352(1):86-99 - PubMed
  31. Cells. 2019 Apr 27;8(5): - PubMed
  32. Fish Shellfish Immunol. 2016 Dec;59:256-267 - PubMed
  33. Autoimmun Rev. 2007 Jan;6(3):155-61 - PubMed
  34. Cell Host Microbe. 2009 May 8;5(5):439-49 - PubMed
  35. Science. 1953 Dec 18;118(3077):733-7 - PubMed
  36. F1000Res. 2017 Nov 07;6:1968 - PubMed
  37. J Fish Dis. 2010 Aug;33(8):701-4 - PubMed
  38. Virol J. 2018 Aug 8;15(1):124 - PubMed
  39. Leuk Lymphoma. 2004 Feb;45(2):257-64 - PubMed
  40. Virol J. 2008 Feb 28;5:36 - PubMed
  41. Nucleic Acids Res. 2015 Jul 1;43(W1):W566-70 - PubMed
  42. Genome Res. 2003 Nov;13(11):2498-504 - PubMed
  43. MBio. 2014 May 13;5(3):e00070-14 - PubMed
  44. Int Immunol. 2009 Apr;21(4):317-37 - PubMed
  45. Fish Shellfish Immunol. 2008 Oct;25(4):351-7 - PubMed
  46. J Virol. 2010 Jul;84(14):7140-50 - PubMed
  47. Proc R Soc Med. 1956 Jan;49(1):55-8 - PubMed
  48. PLoS One. 2011;6(10):e26998 - PubMed
  49. J Virol. 2008 May;82(9):4275-83 - PubMed
  50. Cells. 2018 Apr 19;7(4):null - PubMed
  51. Viruses. 2017 Jun 21;9(6): - PubMed
  52. Biologicals. 2017 Mar;46:6-10 - PubMed
  53. J Virol. 2012 Oct;86(19):10571-8 - PubMed
  54. Histol Histopathol. 1992 Jul;7(3):501-28 - PubMed
  55. Dis Aquat Organ. 2007 Aug 13;77(1):41-52 - PubMed
  56. Rev Med Virol. 2017 Jul;27(4): - PubMed
  57. Nat Immunol. 2009 Oct;10(10):1073-80 - PubMed
  58. Fish Shellfish Immunol. 2019 Apr;87:421-437 - PubMed
  59. Virol J. 2013 Jun 22;10:205 - PubMed
  60. J Virol. 2013 Aug;87(15):8451-64 - PubMed
  61. Vet Res. 2014 Apr 03;45:35 - PubMed
  62. Biochem J. 1995 Jun 1;308 ( Pt 2):613-21 - PubMed
  63. Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12397-401 - PubMed
  64. Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1512-7 - PubMed
  65. Cell Host Microbe. 2012 Oct 18;12(4):432-44 - PubMed
  66. Gene. 2017 Sep 5;627:188-193 - PubMed
  67. J Biol Chem. 2015 Oct 23;290(43):25946-59 - PubMed
  68. Methods. 2001 Dec;25(4):402-8 - PubMed
  69. Fish Shellfish Immunol. 2015 Aug;45(2):780-90 - PubMed
  70. Front Immunol. 2019 May 22;10:1055 - PubMed
  71. J Virol. 2015 Mar;89(5):2462-8 - PubMed
  72. Nat Commun. 2019 Feb 14;10(1):752 - PubMed
  73. Immunity. 2013 Jan 24;38(1):92-105 - PubMed
  74. PLoS One. 2017 Mar 2;12(3):e0173161 - PubMed
  75. Virol J. 2007 Jan 25;4:13 - PubMed
  76. Vet Res. 2015 Mar 06;46:26 - PubMed
  77. Science. 2015 Oct 9;350(6257):217-21 - PubMed
  78. Redox Biol. 2015;4:296-307 - PubMed

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