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Leichner JM, Konyukhov E, Kamoun D, et al. Real time mitochondrial dimension measurements. J Biol Methods. 2019;6(1):e111doi: 10.14440/jbm.2019.262.
Leichner, J. M., Konyukhov, E., Kamoun, D., & Yaniv, Y. (2019). Real time mitochondrial dimension measurements. Journal of biological methods, 6(1), e111. https://doi.org/10.14440/jbm.2019.262
Leichner, Joseph M, et al. "Real time mitochondrial dimension measurements." Journal of biological methods vol. 6,1 (2019): e111. doi: https://doi.org/10.14440/jbm.2019.262
Leichner JM, Konyukhov E, Kamoun D, Yaniv Y. Real time mitochondrial dimension measurements. J Biol Methods. 2019 Mar 27;6(1):e111. doi: 10.14440/jbm.2019.262. eCollection 2019. PMID: 31453260; PMCID: PMC6706126.
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J Biol Methods. 2019 Mar 27;6(1):e111. doi: 10.14440/jbm.2019.262. eCollection 2019.

Real time mitochondrial dimension measurements.

Journal of biological methods

Joseph M Leichner, Evgene Konyukhov, David Kamoun, Yael Yaniv

Affiliations

  1. Biomedical Engineering Faculty, Technion-IIT, Haifa 32000, Israel.

PMID: 31453260 PMCID: PMC6706126 DOI: 10.14440/jbm.2019.262

Abstract

Mitochondrial volume is correlated with cell function and internal cell processes. Changes in mitochondrial volume were associated with advanced states of cardiac disease. Thus, measurements of mitochondrial dimension deformations are important to the understanding of cell function and its deterioration. Existing methods either allow measurements of the volume of isolated mitochondria, which are an inferior model to that of isolated cells, or they allow short time measurements that are toxic to the cells. Recent studies have discovered that mitochondrial deformation along a given cell axis can be measured by using the Fourier transformation on the variation in transmitted light intensity induced by the periodic lattice of myofilaments alternating with mitochondrial rows. However, this method was used only offline and in a line scan mode, making it impossible to measure both axes. We designed an open source program in LabVIEW to take advantage of the transmitted light diffraction technique and quantify mitochondrial two dimension (2D) deformation in cardiomyocytes,

Keywords: energetics; mitochondrial deformation; mitochondrial volume; real time; sarcomere length

Conflict of interest statement

Competing interests: The authors have declared that no competing interests exist.

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