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Zuvin M, Kuruoglu E, Kaya VO, et al. Magnetofection of Green Fluorescent Protein Encoding DNA-Bearing Polyethyleneimine-Coated Superparamagnetic Iron Oxide Nanoparticles to Human Breast Cancer Cells. ACS Omega. 2019;4(7):12366-12374doi: 10.1021/acsomega.9b01000.
Zuvin, M., Kuruoglu, E., Kaya, V. O., Unal, O., Kutlu, O., Yagci Acar, H., Gozuacik, D., & Koşar, A. (2019). Magnetofection of Green Fluorescent Protein Encoding DNA-Bearing Polyethyleneimine-Coated Superparamagnetic Iron Oxide Nanoparticles to Human Breast Cancer Cells. ACS omega, 4(7), 12366-12374. https://doi.org/10.1021/acsomega.9b01000
Zuvin, Merve, et al. "Magnetofection of Green Fluorescent Protein Encoding DNA-Bearing Polyethyleneimine-Coated Superparamagnetic Iron Oxide Nanoparticles to Human Breast Cancer Cells." ACS omega vol. 4,7 (2019): 12366-12374. doi: https://doi.org/10.1021/acsomega.9b01000
Zuvin M, Kuruoglu E, Kaya VO, Unal O, Kutlu O, Yagci Acar H, Gozuacik D, Koşar A. Magnetofection of Green Fluorescent Protein Encoding DNA-Bearing Polyethyleneimine-Coated Superparamagnetic Iron Oxide Nanoparticles to Human Breast Cancer Cells. ACS Omega. 2019 Jul 18;4(7):12366-12374. doi: 10.1021/acsomega.9b01000. eCollection 2019 Jul 31. PMID: 31460354; PMCID: PMC6682024.
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ACS Omega. 2019 Jul 18;4(7):12366-12374. doi: 10.1021/acsomega.9b01000. eCollection 2019 Jul 31.

Magnetofection of Green Fluorescent Protein Encoding DNA-Bearing Polyethyleneimine-Coated Superparamagnetic Iron Oxide Nanoparticles to Human Breast Cancer Cells.

ACS omega

Merve Zuvin, Efe Kuruoglu, Veysel Ogulcan Kaya, Ozlem Unal, Ozlem Kutlu, Havva Yagci Acar, Devrim Gozuacik, Ali Koşar

Affiliations

  1. Mechatronics Engineering Program, Faculty of Engineering and Natural Sciences, Molecular Biology, Genetics and Bioengineering Program, Faculty of Engineering and Natural Sciences, and Center of Excellence for Functional Surfaces and Interfaces for Nano Diagnostics (EFSUN), Sabanci University, Orhanli, 34956 Tuzla, Istanbul, Turkey.
  2. Department of Chemistry, Faculty of Arts and Sciences, Koc University, 34450 Sariyer, Istanbul, Turkey.
  3. SUNUM Nanotechnology Research and Application Center, Orhanli, 34956 Tuzla, Istanbul, Turkey.

PMID: 31460354 PMCID: PMC6682024 DOI: 10.1021/acsomega.9b01000

Abstract

Gene therapy is a developing method for the treatment of various diseases. For this purpose, the search for nonviral methods has recently accelerated to avoid toxic effects. A strong alternative method is magnetofection, which involves the use of superparamagnetic iron oxide nanoparticles (SPIONs) with a proper organic coating and external magnetic field to enhance the localization of SPIONs at the target site. In this study, a new magnetic actuation system consisting of four rare-earth magnets on a rotary table was designed and manufactured to obtain improved magnetofection. As a model, green fluorescent protein DNA-bearing polyethyleneimine-coated SPIONs were used. Magnetofection was tested on MCF7 cells. The system reduced the transfection time (down to 1 h) of the standard polyethyleneimine transfection protocol. As a result, we showed that the system could be effectively used for gene transfer.

Conflict of interest statement

The authors declare no competing financial interest.

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