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Aper T, Wilhelmi M, Boer U, et al. Dehydration improves biomechanical strength of bioartificial vascular graft material and allows its long-term storage. Innov Surg Sci. 2018;3(3):215-224doi: 10.1515/iss-2018-0017.
Aper, T., Wilhelmi, M., Boer, U., Lau, S., Benecke, N., Hilfiker, A., & Haverich, A. (2018). Dehydration improves biomechanical strength of bioartificial vascular graft material and allows its long-term storage. Innovative surgical sciences, 3(3), 215-224. https://doi.org/10.1515/iss-2018-0017
Aper, Thomas, et al. "Dehydration improves biomechanical strength of bioartificial vascular graft material and allows its long-term storage." Innovative surgical sciences vol. 3,3 (2018): 215-224. doi: https://doi.org/10.1515/iss-2018-0017
Aper T, Wilhelmi M, Boer U, Lau S, Benecke N, Hilfiker A, Haverich A. Dehydration improves biomechanical strength of bioartificial vascular graft material and allows its long-term storage. Innov Surg Sci. 2018 Jul 23;3(3):215-224. doi: 10.1515/iss-2018-0017. eCollection 2018 Sep. PMID: 31579785; PMCID: PMC6604580.
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Innov Surg Sci. 2018 Jul 23;3(3):215-224. doi: 10.1515/iss-2018-0017. eCollection 2018 Sep.

Dehydration improves biomechanical strength of bioartificial vascular graft material and allows its long-term storage.

Innovative surgical sciences

Thomas Aper, Mathias Wilhelmi, Ulrike Boer, Skadi Lau, Nils Benecke, Andres Hilfiker, Axel Haverich

Affiliations

  1. Department of Vascular and Endovascular Surgery, Division for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.

PMID: 31579785 PMCID: PMC6604580 DOI: 10.1515/iss-2018-0017

Abstract

INTRODUCTION: We have recently reported about a novel technique for the generation of bioartificial vascular grafts based on the use of a compacted fibrin matrix. In this study, we evaluated the effects of a dehydration process on the biomechanical properties of compacted fibrin tubes and whether it allows for their long-term storage.

MATERIALS AND METHODS: Fibrin was precipitated from fresh frozen plasma by means of cryoprecipitation and simultaneously with a thrombin solution applied in a high-speed rotating casting mold. Subsequent dehydration of the fibrin tubes (29/38) was performed in dry air with a dilator inside the tube to prevent the collapse of the lumen. Dehydrated fibrin tubes were stored for six (n=9) and 12 months (n=10) at room temperature. Comparative analysis was done on initially generated and dehydrated fibrin tubes before and after storage to evaluate the effects of the dehydration process and storage on the biomechanical properties and structure of the tubes.

RESULTS: Thirty-eight fibrin tubes were generated by high-speed rotation-molding from 142±3 mg fibrinogen with an inner diameter of 5.8±0.1 mm and a length of 100 mm. A centrifugal force of nearly 900×

DISCUSSION: Compaction of a fibrin matrix in high-speed rotation-moulding and subsequent dehydration enables for the construction of small-caliber fibrin grafts. Over and above, the dehydration process allows their storage and stockpiling as a prerequisite for clinical use.

©2018 Aper T., et al., published by De Gruyter, Berlin/Boston.

Keywords: dehydration; fibrin; tissue engineering; vascular graft

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