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Michaelis M, Fayyaz A, Parambath M, et al. Platform for Screening Abiotic/Biotic Interactions Using Indicator Displacement Assays. Langmuir. 2019;35(44):14230-14237doi: 10.1021/acs.langmuir.9b03085.
Michaelis, M., Fayyaz, A., Parambath, M., Koeppen, S., Ciacchi, L. C., Hanley, Q. S., & Perry, C. C. (2019). Platform for Screening Abiotic/Biotic Interactions Using Indicator Displacement Assays. Langmuir : the ACS journal of surfaces and colloids, 35(44), 14230-14237. https://doi.org/10.1021/acs.langmuir.9b03085
Michaelis, Monika, et al. "Platform for Screening Abiotic/Biotic Interactions Using Indicator Displacement Assays." Langmuir : the ACS journal of surfaces and colloids vol. 35,44 (2019): 14230-14237. doi: https://doi.org/10.1021/acs.langmuir.9b03085
Michaelis M, Fayyaz A, Parambath M, Koeppen S, Ciacchi LC, Hanley QS, Perry CC. Platform for Screening Abiotic/Biotic Interactions Using Indicator Displacement Assays. Langmuir. 2019 Nov 05;35(44):14230-14237. doi: 10.1021/acs.langmuir.9b03085. Epub 2019 Oct 25. PMID: 31609123.
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Langmuir. 2019 Nov 05;35(44):14230-14237. doi: 10.1021/acs.langmuir.9b03085. Epub 2019 Oct 25.

Platform for Screening Abiotic/Biotic Interactions Using Indicator Displacement Assays.

Langmuir : the ACS journal of surfaces and colloids

Monika Michaelis, Aneeqa Fayyaz, Mithun Parambath, Susan Koeppen, Lucio Colombi Ciacchi, Quentin S Hanley, Carole C Perry

Affiliations

  1. Hybrid Materials Interfaces Group, Faculty of Production Engineering, Bremen Center for Computational Material Science (BCCMS), Center for Environmental Research and Sustainable Technology (UFT) and MAPEX Centre for Materials and Processes , University of Bremen , D-28359 Bremen , Germany.

PMID: 31609123 DOI: 10.1021/acs.langmuir.9b03085

Abstract

This paper describes novel adaptations of optically sectioned planar format assays to screen compounds for their affinities to materials surfaces. The novel platform, which we name optically sectioned indicator displacement assays (O-IDA), makes use of displaceable dyes in a format adaptable to high-throughput multiwell plate technologies. We describe two approaches: the first being where the dye exhibits fluorescence in both the surface bound and unbound state and the second, where fluorescence is lost upon displacement of the dye from the surface. Half maximal inhibitory concentration (IC

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