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Medina NP, De Guia ACM, Venturina VM, et al. Molecular characterization of MHC II DRB3 gene of swamp- and riverine-type water buffaloes. J Adv Vet Anim Res. 2019;6(3):308-314doi: 10.5455/javar.2019.f349.
Medina, N. P., De Guia, A. C. M., Venturina, V. M., & Mingala, C. N. (2019). Molecular characterization of MHC II DRB3 gene of swamp- and riverine-type water buffaloes. Journal of advanced veterinary and animal research, 6(3), 308-314. https://doi.org/10.5455/javar.2019.f349
Medina, Noraine P, et al. "Molecular characterization of MHC II DRB3 gene of swamp- and riverine-type water buffaloes." Journal of advanced veterinary and animal research vol. 6,3 (2019): 308-314. doi: https://doi.org/10.5455/javar.2019.f349
Medina NP, De Guia ACM, Venturina VM, Mingala CN. Molecular characterization of MHC II DRB3 gene of swamp- and riverine-type water buffaloes. J Adv Vet Anim Res. 2019 Jul 11;6(3):308-314. doi: 10.5455/javar.2019.f349. eCollection 2019 Sep. PMID: 31583227; PMCID: PMC6760514.
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J Adv Vet Anim Res. 2019 Jul 11;6(3):308-314. doi: 10.5455/javar.2019.f349. eCollection 2019 Sep.

Molecular characterization of MHC II DRB3 gene of swamp- and riverine-type water buffaloes.

Journal of advanced veterinary and animal research

Noraine P Medina, Arren Christian M De Guia, Virginia M Venturina, Claro N Mingala

Affiliations

  1. College of Veterinary Science and Medicine, Central Luzon State University, Science City of Munoz, Nueva Ecija, The Philippines.
  2. Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Nueva Ecija, The Philippines.
  3. Philippine Carabao Center National Headquarters and Gene Pool, Maharlika Highway, Science City of Munoz 3120, Nueva Ecija, The Philippines.

PMID: 31583227 PMCID: PMC6760514 DOI: 10.5455/javar.2019.f349

Abstract

OBJECTIVE: Major histocompatibility complex (MHC) is a set of molecular proteins on the surface of antigen presenting cells encoded by a large gene family which are important parts of the immune system. This study was conducted to convey information on the genetic characteristics of the MHC II DRB3 gene in riverine and swamp buffaloes.

MATERIALS AND METHODS: Characterization of MHC II DRB3 gene was carried out using polymerase chain reaction (PCR)-based assay. Thirty-milliliter milk samples were collected from 10 swamp-type and 10 riverine-type buffaloes. RNA from milk samples were extracted using Trizol and then followed by reverse transcription-PCR (RT-PCR).

RESULTS: The phylogenetic analysis with 1,000 bootstrap replications clearly showed complex parsimony in MHC II DRB3 gene between 10 riverine- and 10 swamp-type but also confirmed that the samples are similar to

CONCLUSION: Based on the molecular data, the current study conforms to other works of literature that this gene is highly polymorphic which can be due to its function in the immune responsiveness and disease resistance. Further study on the immunological response of MHC II DRB3 to infection may elucidate its underlying function and role in the protection against specific disease of animals.

Copyright: © Journal of Advanced Veterinary and Animal Research.

Keywords: MHC II DRB3; phylogenetic analysis; water buffaloes

Conflict of interest statement

The authors declare that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript.

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