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Chen Y, Rofidal V, Hem S, et al. Targeted Proteomics Allows Quantification of Ethylene Receptors and Reveals SlETR3 Accumulation in Never-Ripe Tomatoes. Front Plant Sci. 2019;10:1054doi: 10.3389/fpls.2019.01054.
Chen, Y., Rofidal, V., Hem, S., Gil, J., Nosarzewska, J., Berger, N., Demolombe, V., Bouzayen, M., Azhar, B. J., Shakeel, S. N., Schaller, G. E., Binder, B. M., Santoni, V., Chervin, C., Fuchs, L. S., & Fuchs, D. (2019). Targeted Proteomics Allows Quantification of Ethylene Receptors and Reveals SlETR3 Accumulation in Never-Ripe Tomatoes. Frontiers in plant science, 101054. https://doi.org/10.3389/fpls.2019.01054
Chen, Yi, et al. "Targeted Proteomics Allows Quantification of Ethylene Receptors and Reveals SlETR3 Accumulation in Never-Ripe Tomatoes." Frontiers in plant science vol. 10 (2019): 1054. doi: https://doi.org/10.3389/fpls.2019.01054
Chen Y, Rofidal V, Hem S, Gil J, Nosarzewska J, Berger N, Demolombe V, Bouzayen M, Azhar BJ, Shakeel SN, Schaller GE, Binder BM, Santoni V, Chervin C, Fuchs LS, Fuchs D. Targeted Proteomics Allows Quantification of Ethylene Receptors and Reveals SlETR3 Accumulation in Never-Ripe Tomatoes. Front Plant Sci. 2019 Aug 29;10:1054. doi: 10.3389/fpls.2019.01054. eCollection 2019. PMID: 31555314; PMCID: PMC6727826.
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Front Plant Sci. 2019 Aug 29;10:1054. doi: 10.3389/fpls.2019.01054. eCollection 2019.

Targeted Proteomics Allows Quantification of Ethylene Receptors and Reveals SlETR3 Accumulation in Never-Ripe Tomatoes.

Frontiers in plant science

Yi Chen, Valérie Rofidal, Sonia Hem, Julie Gil, Joanna Nosarzewska, Nathalie Berger, Vincent Demolombe, Mondher Bouzayen, Beenish J Azhar, Samina N Shakeel, G Eric Schaller, Brad M Binder, Véronique Santoni, Christian Chervin, Fuchs, Fuchs

Affiliations

  1. GBF, Université de Toulouse, INRA, Toulouse, France.
  2. BPMP, CNRS, INRA, Montpellier SupAgro, Université de Montpellier, Montpellier, France.
  3. Department of Biochemistry, Quaid-i-azam University, Islamabad, Pakistan.
  4. Department of Biological Sciences, Dartmouth College, Hanover, NH, United States.
  5. Department of Biochemistry, Cellular, and Molecular Biology, University of Tennessee, Knoxville, TN, United States.

PMID: 31555314 PMCID: PMC6727826 DOI: 10.3389/fpls.2019.01054

Abstract

Ethylene regulates fruit ripening and several plant functions (germination, plant growth, plant-microbe interactions). Protein quantification of ethylene receptors (ETRs) is essential to study their functions, but is impaired by low resolution tools such as antibodies that are mostly nonspecific, or the lack of sensitivity of shotgun proteomic approaches. We developed a targeted proteomic method, to quantify low-abundance proteins such as ETRs, and coupled this to mRNAs analyses, in two tomato lines: Wild Type (WT) and Never-Ripe (NR) which is insensitive to ethylene because of a gain-of-function mutation in ETR3. We obtained mRNA and protein abundance profiles for each ETR over the fruit development period. Despite a limiting number of replicates, we propose Pearson correlations between mRNA and protein profiles as interesting indicators to discriminate the two genotypes: such correlations are mostly positive in the WT and are affected by the NR mutation. The influence of putative post-transcriptional and post-translational changes are discussed. In NR fruits, the observed accumulation of the mutated ETR3 protein between ripening stages (Mature Green and Breaker + 8 days) may be a cause of NR tomatoes to stay orange. The label-free quantitative proteomics analysis of membrane proteins, concomitant to Parallel Reaction Monitoring analysis, may be a resource to study changes over tomato fruit development. These results could lead to studies about ETR subfunctions and interconnections over fruit development. Variations of RNA-protein correlations may open new fields of research in ETR regulation. Finally, similar approaches may be developed to study ETRs in whole plant development and plant-microorganism interactions.

Keywords: ethylene; hormone; receptor; signaling; tomato

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