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Fletcher PA, Smiljanic K, Maso Prévide R, et al. Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells. Front Endocrinol (Lausanne). 2019;10:623doi: 10.3389/fendo.2019.00623.
Fletcher, P. A., Smiljanic, K., Maso Prévide, R., Iben, J. R., Li, T., Rokic, M. B., Sherman, A., Coon, S. L., & Stojilkovic, S. S. (2019). Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells. Frontiers in endocrinology, 10623. https://doi.org/10.3389/fendo.2019.00623
Fletcher, Patrick A, et al. "Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells." Frontiers in endocrinology vol. 10 (2019): 623. doi: https://doi.org/10.3389/fendo.2019.00623
Fletcher PA, Smiljanic K, Maso Prévide R, Iben JR, Li T, Rokic MB, Sherman A, Coon SL, Stojilkovic SS. Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells. Front Endocrinol (Lausanne). 2019 Sep 18;10:623. doi: 10.3389/fendo.2019.00623. eCollection 2019. PMID: 31620083; PMCID: PMC6760010.
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Front Endocrinol (Lausanne). 2019 Sep 18;10:623. doi: 10.3389/fendo.2019.00623. eCollection 2019.

Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells.

Frontiers in endocrinology

Patrick A Fletcher, Kosara Smiljanic, Rafael Maso Prévide, James R Iben, Tianwei Li, Milos B Rokic, Arthur Sherman, Steven L Coon, Stanko S Stojilkovic

Affiliations

  1. Laboratory of Biological Modeling, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health (NIH), Bethesda, MD, United States.
  2. Section on Cellular Signaling, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health (NIH), Bethesda, MD, United States.
  3. Molecular Genomics Core, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health (NIH), Bethesda, MD, United States.

PMID: 31620083 PMCID: PMC6760010 DOI: 10.3389/fendo.2019.00623

Abstract

Understanding the physiology and pathology of an organ composed of a variety of cell populations depends critically on genome-wide information on each cell type. Here, we report single-cell transcriptome profiling of over 6,800 freshly dispersed anterior pituitary cells from postpubertal male and female rats. Six pituitary-specific cell types were identified based on known marker genes and characterized: folliculostellate cells and hormone-producing corticotrophs, gonadotrophs, thyrotrophs, somatotrophs, and lactotrophs. Also identified were endothelial and blood cells from the pituitary capillary network. The expression of numerous developmental and neuroendocrine marker genes in both folliculostellate and hormone-producing cells supports that they have a common origin. For several genes, the validity of transcriptome analysis was confirmed by qRT-PCR and single cell immunocytochemistry. Folliculostellate cells exhibit impressive transcriptome diversity, indicating their major roles in production of endogenous ligands and detoxification enzymes, and organization of extracellular matrix. Transcriptome profiles of hormone-producing cells also indicate contributions toward those functions, while also clearly demonstrating their endocrine function. This survey highlights many novel genetic markers contributing to pituitary cell type identity, sexual dimorphism, and function, and points to relationships between hormone-producing and folliculostellate cells.

Copyright © 2019 Fletcher, Smiljanic, Maso Prévide, Iben, Li, Rokic, Sherman, Coon and Stojilkovic.

Keywords: folliculostellate cells; hormone-producing cells; pituitary gland; rat; sexual dimorphism; single-cell RNA sequencing; transcriptome

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