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Karami A, Tebyanian H, Sayyad Soufdoost R, et al. Extraction and Characterization of Collagen with Cost-Effective Method from Human Placenta for Biomedical Applications. World J Plast Surg. 2019;8(3):352-358doi: 10.29252/wjps.8.3.352.
Karami, A., Tebyanian, H., Sayyad Soufdoost, R., Motavallian, E., Barkhordari, A., & Nourani, M. R. (2019). Extraction and Characterization of Collagen with Cost-Effective Method from Human Placenta for Biomedical Applications. World journal of plastic surgery, 8(3), 352-358. https://doi.org/10.29252/wjps.8.3.352
Karami, Ali, et al. "Extraction and Characterization of Collagen with Cost-Effective Method from Human Placenta for Biomedical Applications." World journal of plastic surgery vol. 8,3 (2019): 352-358. doi: https://doi.org/10.29252/wjps.8.3.352
Karami A, Tebyanian H, Sayyad Soufdoost R, Motavallian E, Barkhordari A, Nourani MR. Extraction and Characterization of Collagen with Cost-Effective Method from Human Placenta for Biomedical Applications. World J Plast Surg. 2019 Sep;8(3):352-358. doi: 10.29252/wjps.8.3.352. PMID: 31620338; PMCID: PMC6790260.
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World J Plast Surg. 2019 Sep;8(3):352-358. doi: 10.29252/wjps.8.3.352.

Extraction and Characterization of Collagen with Cost-Effective Method from Human Placenta for Biomedical Applications.

World journal of plastic surgery

Ali Karami, Hamid Tebyanian, Reza Sayyad Soufdoost, Ebrahim Motavallian, Aref Barkhordari, Mohammad Reza Nourani

Affiliations

  1. Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
  2. Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran.
  3. Imam Khomeini Clinic of Dentistry, Tehran, Iran.
  4. Department of General Surgery, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.

PMID: 31620338 PMCID: PMC6790260 DOI: 10.29252/wjps.8.3.352

Abstract

BACKGROUND: Collagen is the main product in pharmaceutics and food industry with a high demand. Collagen can be extracted from several tissues such as skin, bone and tendon, etc. Collagen can be used in tissue engineering researches as a substrate of wound healing and nerve regeneration. Extraction methods of collagen are various with different purities. In this research, we aimed to extract collagen from human placenta with a modified method.

METHODS: This modified approach was used for extracting of collagen from human placenta with acetic acid and NaCl treatment using different concentrations.

RESULTS: SDS page showed three different bands that reflected two alpha-chains and one beta-chain with molecular weights of 102, 118 and 220 kDa, respectively. There was no significant difference between extracted collagen from human placenta and standard collagen in western blot analysis.

CONCLUSION: It was concluded that human placenta can be an alternative source of collagen with high purity for biomedical applications such as tissue engineering, stem cell therapy and research.

Keywords: Characterization; Collagen; Extraction; Huma; Placenta

Conflict of interest statement

The authors declare no conflict of interest.

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