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Havelund JF, Nygaard KH, Nielsen TH, et al. In Vivo Microdialysis of Endogenous and . Metabolites. 2019;9(10)doi: 10.3390/metabo9100204.
Havelund, J. F., Nygaard, K. H., Nielsen, T. H., Nordström, C. H., Poulsen, F. R., Færgeman, N. J., Forsse, A., & Gramsbergen, J. B. (2019). In Vivo Microdialysis of Endogenous and . Metabolites, 9(10), . https://doi.org/10.3390/metabo9100204
Havelund, Jesper F, et al. "In Vivo Microdialysis of Endogenous and ." Metabolites vol. 9,10 (2019). doi: https://doi.org/10.3390/metabo9100204
Havelund JF, Nygaard KH, Nielsen TH, Nordström CH, Poulsen FR, Færgeman NJ, Forsse A, Gramsbergen JB. In Vivo Microdialysis of Endogenous and . Metabolites. 2019 Sep 27;9(10). doi: 10.3390/metabo9100204. PMID: 31569792; PMCID: PMC6835622.
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Metabolites. 2019 Sep 27;9(10). doi: 10.3390/metabo9100204.

In Vivo Microdialysis of Endogenous and .

Metabolites

Jesper F Havelund, Kevin H Nygaard, Troels H Nielsen, Carl-Henrik Nordström, Frantz R Poulsen, Nils J Færgeman, Axel Forsse, Jan Bert Gramsbergen

Affiliations

  1. VILLUM Center for Bioanalytical Sciences, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark. [email protected].
  2. Department of Neurosurgery, Odense University Hospital, University of Southern Denmark, Sdr. Boulevard 29, 5000 Odense C, Denmark. [email protected].
  3. BRIDGE-Brain ResearchE-Inter-Disciplinary Guided Excellence, Institute of Clinical Research, University of Southern Denmark, Winsløwparken 19, 5000 Odense C, Denmark. [email protected].
  4. Department of Neurosurgery, Odense University Hospital, University of Southern Denmark, Sdr. Boulevard 29, 5000 Odense C, Denmark. [email protected].
  5. BRIDGE-Brain ResearchE-Inter-Disciplinary Guided Excellence, Institute of Clinical Research, University of Southern Denmark, Winsløwparken 19, 5000 Odense C, Denmark. [email protected].
  6. Department of Neurosurgery, Odense University Hospital, University of Southern Denmark, Sdr. Boulevard 29, 5000 Odense C, Denmark. [email protected].
  7. BRIDGE-Brain ResearchE-Inter-Disciplinary Guided Excellence, Institute of Clinical Research, University of Southern Denmark, Winsløwparken 19, 5000 Odense C, Denmark. [email protected].
  8. Department of Neurosurgery, Odense University Hospital, University of Southern Denmark, Sdr. Boulevard 29, 5000 Odense C, Denmark. [email protected].
  9. BRIDGE-Brain ResearchE-Inter-Disciplinary Guided Excellence, Institute of Clinical Research, University of Southern Denmark, Winsløwparken 19, 5000 Odense C, Denmark. [email protected].
  10. VILLUM Center for Bioanalytical Sciences, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark. [email protected].
  11. Department of Neurosurgery, Odense University Hospital, University of Southern Denmark, Sdr. Boulevard 29, 5000 Odense C, Denmark. [email protected].
  12. BRIDGE-Brain ResearchE-Inter-Disciplinary Guided Excellence, Institute of Clinical Research, University of Southern Denmark, Winsløwparken 19, 5000 Odense C, Denmark. [email protected].
  13. Institute of Molecular Medicine, University of Southern Denmark, 55, 5230 Odense C, Denmark. [email protected].

PMID: 31569792 PMCID: PMC6835622 DOI: 10.3390/metabo9100204

Abstract

Cerebral micro-dialysis allows continuous sampling of extracellular metabolites, including glucose, lactate and pyruvate. Transient ischemic events cause a rapid drop in glucose and a rise in lactate levels. Following such events, the lactate/pyruvate (L/P) ratio may remain elevated for a prolonged period of time. In neurointensive care clinics, this ratio is considered a metabolic marker of ischemia and/or mitochondrial dysfunction. Here we propose a novel, sensitive microdialysis liquid chromatography-mass spectrometry (LC-MS) approach to monitor mitochondrial dysfunction in living brain using perfusion with

Keywords: 13C-labeled succinate; LC-MS; cerebral ischemia; endothelin-1; energy metabolism; malonate; micro-dialysis; mitochondrial dysfunction; reperfusion; tricarboxylic acid cycle

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