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J Biol Eng. 2019 Oct 29;13:81. doi: 10.1186/s13036-019-0215-y. eCollection 2019.

Unique .

Journal of biological engineering

Junyan Ma, Qian Li, Haidong Tan, Hao Jiang, Kuikui Li, Lihua Zhang, Quan Shi, Heng Yin

Affiliations

  1. 1Natural Products and Glyco-Biotechnology Research Group, Liaoning Province Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023 China.
  2. 2Liaoning Province Key Laboratory of Bio-Organic Chemistry, Dalian University, Dalian, 116622 China.
  3. 3Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023 China.

PMID: 31737090 PMCID: PMC6844067 DOI: 10.1186/s13036-019-0215-y

Abstract

BACKGROUND: Inulinase can hydrolyze polyfructan into high-fructose syrups and fructoligosaccharides, which are widely used in food, the medical industry and the biorefinery of

RESULTS: Five

CONCLUSION: The increased enzyme activity in N362Q, combined with thermostability testing, 3D modeling, kinetics data and secondary structure analysis, implied that the

© The Author(s). 2019.

Keywords: Enzyme activity; Exo-inulinase; N-glycosylation; Thermostability; β-Sandwich domain

Conflict of interest statement

Competing interestsThe authors declare that they have no competing interests.

References

  1. J Biol Chem. 2013 Aug 30;288(35):25522-34 - PubMed
  2. Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):20889-94 - PubMed
  3. J Biol Chem. 2015 Apr 24;290(17):10572-86 - PubMed
  4. Biochimie. 2017 Jan;132:102-108 - PubMed
  5. Proc Natl Acad Sci U S A. 1995 Jan 3;92(1):97-101 - PubMed
  6. BMB Rep. 2014 May;47(5):256-61 - PubMed
  7. Appl Environ Microbiol. 2014 Jul;80(13):3962-71 - PubMed
  8. J Biol Chem. 2012 May 4;287(19):15906-15 - PubMed
  9. Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3131-6 - PubMed
  10. PLoS One. 2015 Apr 07;10(4):e0120601 - PubMed
  11. J Biol Chem. 2017 Oct 13;292(41):16955-16968 - PubMed
  12. Chem Biol. 1998 Aug;5(8):427-37 - PubMed
  13. J Am Chem Soc. 2017 Sep 20;139(37):12947-12955 - PubMed
  14. J Biol Chem. 2013 Apr 5;288(14):9755-66 - PubMed
  15. Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4334-9 - PubMed
  16. Bioprocess Biosyst Eng. 2015 Feb;38(2):263-72 - PubMed
  17. BMC Microbiol. 2010 Feb 16;10:48 - PubMed
  18. Biochimie. 2014 Jun;101:39-49 - PubMed
  19. Biochemistry. 2017 Jul 5;56(26):3380-3393 - PubMed
  20. Int J Biol Macromol. 2017 May;98:542-549 - PubMed
  21. Biochemistry. 2014 Aug 5;53(30):4884-93 - PubMed
  22. New Phytol. 2007;176(2):317-24 - PubMed
  23. J Biol Chem. 2010 Apr 30;285(18):13930-41 - PubMed
  24. Biotechnol Bioeng. 2016 Feb;113(2):283-91 - PubMed
  25. Proc Natl Acad Sci U S A. 2014 May 27;111(21):7612-7 - PubMed
  26. Microb Cell Fact. 2015 Mar 21;14:40 - PubMed
  27. Gene. 2013 Mar 10;516(2):255-62 - PubMed
  28. Talanta. 2016;146:225-30 - PubMed
  29. J Sci Food Agric. 2018 May;98(7):2540-2547 - PubMed
  30. J Agric Food Chem. 2017 Jul 26;65(29):6009-6015 - PubMed
  31. Protein Eng Des Sel. 2017 Jul 1;30(7):495-501 - PubMed
  32. Curr Opin Chem Biol. 1999 Dec;3(6):643-9 - PubMed
  33. Appl Biochem Biotechnol. 2016 Jan;178(1):144-58 - PubMed
  34. Microbes Environ. 2010;25(3):152-5 - PubMed
  35. J Biol Chem. 2016 Mar 25;291(13):6843-57 - PubMed
  36. Bioconjug Chem. 2011 Mar 16;22(3):488-96 - PubMed
  37. Mol Biotechnol. 2015 Apr;57(4):337-47 - PubMed
  38. Biotechnol Bioeng. 2013 Dec;110(12):3075-84 - PubMed
  39. J Biomol Struct Dyn. 2012;29(5):905-20 - PubMed
  40. Bioresour Technol. 2011 Mar;102(6):4295-303 - PubMed
  41. J Biotechnol. 2014 Feb 10;171:3-7 - PubMed
  42. Structure. 2015 Sep 1;23(9):1573-1583 - PubMed
  43. Sci Rep. 2017 Jul 5;7(1):4656 - PubMed
  44. Int J Biol Macromol. 2018 Jun;112:490-498 - PubMed
  45. J Biosci Bioeng. 2015 Mar;119(3):267-74 - PubMed
  46. Plant Physiol Biochem. 2017 Oct;119:200-210 - PubMed
  47. Biotechnol Lett. 2018 May;40(5):847-854 - PubMed
  48. J Biol Chem. 2012 Jun 1;287(23):19674-86 - PubMed

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