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Zhang L, Zhuang Z, Sun Y, et al. Velvet antler polypeptide is able to induce differentiation of neural stem cells towards neurons in vitro. J Tradit Chin Med. 2017;37(3):308-313
Zhang, L., Zhuang, Z., Sun, Y., Ma, S., Yang, W., Lei, H., Ouyang, J., Wang, Y., & Zuo, J. (2017). Velvet antler polypeptide is able to induce differentiation of neural stem cells towards neurons in vitro. Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan, 37(3), 308-313.
Zhang, Lihong, et al. "Velvet antler polypeptide is able to induce differentiation of neural stem cells towards neurons in vitro." Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan vol. 37,3 (2017): 308-313.
Zhang L, Zhuang Z, Sun Y, Ma S, Yang W, Lei H, Ouyang J, Wang Y, Zuo J. Velvet antler polypeptide is able to induce differentiation of neural stem cells towards neurons in vitro. J Tradit Chin Med. 2017 Jun;37(3):308-313. PMID: 31682372.
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J Tradit Chin Med. 2017 Jun;37(3):308-313.

Velvet antler polypeptide is able to induce differentiation of neural stem cells towards neurons in vitro.

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan

Lihong Zhang, Zhihong Zhuang, Yanan Sun, Shuhua Ma, Weifeng Yang, Hongtao Lei, Jingfeng Ouyang, Yi Wang, Junling Zuo

Affiliations

  1. Graduate School, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
  2. Morphological Laboratory, Expeimental Research Center, Research and Development Service Base, China Academy of Chinese Medical Sciences, Beijing 100700, China.
  3. First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.

PMID: 31682372

Abstract

OBJECTIVE: To investigate the neural differentiation capacity of water extraction of velvet antler.

METHODS: Velvet antler (Cervus Nippon Temminck) polypeptide (VAP) was used to differentiate neural stem cells (NSCs) towards neurons in the study. Firstly, we obtain the polypeptides of VAP by water extraction. Secondly, we observed the morphology, assayed the factors in the media by enzyme-linked immunosorbent assay, and detected the special neural molecules by immunfluorescence staining. NSCs were cultured on the cell climbing film. After neuronal differentiation, differentiated NSCs were mounted for immunocytochemistry with immunofluorescence technique.

RESULTS: The differentiating cells look like neuron, some special factors, such as Glial cell line-derived neurotrophic factor, nerve growth factor, in the media can be detected while differentiated neuron-like cells can express the special neural molecules.

CONCLUSION: Differentiation of NSCs towards neurons can be induced by velvet antler polypeptide.

Keywords: Cornu cervi pantotrichum; Glial cell line-derived neurotrophic factors, Nerve growth factor; Neural stem cell

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