Display options
Cite
Vallejo R, Platt DC, Rink JA, et al. Electrical Stimulation of C6 Glia-Precursor Cells In Vitro Differentially Modulates Gene Expression Related to Chronic Pain Pathways. Brain Sci. 2019;9(11)doi: 10.3390/brainsci9110303.
Vallejo, R., Platt, D. C., Rink, J. A., Jones, M. A., Kelley, C. A., Gupta, A., Cass, C. L., Eichenberg, K., Vallejo, A., Smith, W. J., Benyamin, R., & Cedeño, D. L. (2019). Electrical Stimulation of C6 Glia-Precursor Cells In Vitro Differentially Modulates Gene Expression Related to Chronic Pain Pathways. Brain sciences, 9(11), . https://doi.org/10.3390/brainsci9110303
Vallejo, Ricardo, et al. "Electrical Stimulation of C6 Glia-Precursor Cells In Vitro Differentially Modulates Gene Expression Related to Chronic Pain Pathways." Brain sciences vol. 9,11 (2019). doi: https://doi.org/10.3390/brainsci9110303
Vallejo R, Platt DC, Rink JA, Jones MA, Kelley CA, Gupta A, Cass CL, Eichenberg K, Vallejo A, Smith WJ, Benyamin R, Cedeño DL. Electrical Stimulation of C6 Glia-Precursor Cells In Vitro Differentially Modulates Gene Expression Related to Chronic Pain Pathways. Brain Sci. 2019 Oct 31;9(11). doi: 10.3390/brainsci9110303. PMID: 31683631; PMCID: PMC6896182.
Copy Download .nbib Format: NLM
Share it on

Brain Sci. 2019 Oct 31;9(11). doi: 10.3390/brainsci9110303.

Electrical Stimulation of C6 Glia-Precursor Cells In Vitro Differentially Modulates Gene Expression Related to Chronic Pain Pathways.

Brain sciences

Ricardo Vallejo, David C Platt, Jonathan A Rink, Marjorie A Jones, Courtney A Kelley, Ashim Gupta, Cynthia L Cass, Kirk Eichenberg, Alejandro Vallejo, William J Smith, Ramsin Benyamin, David L Cedeño

Affiliations

  1. Millennium Pain Center, Bloomington, IL 61704, USA. [email protected].
  2. Department of Psychology, Illinois Wesleyan University, Bloomington, IL 61701, USA. [email protected].
  3. Department of Chemistry, Illinois State University, Normal, IL 61790, USA. [email protected].
  4. Department of Biology, Illinois Wesleyan University, Bloomington, IL 61701, USA. [email protected].
  5. Department of Chemistry, Illinois State University, Normal, IL 61790, USA. [email protected].
  6. Millennium Pain Center, Bloomington, IL 61704, USA. [email protected].
  7. Department of Psychology, Illinois Wesleyan University, Bloomington, IL 61701, USA. [email protected].
  8. Millennium Pain Center, Bloomington, IL 61704, USA. [email protected].
  9. Department of Psychology, Illinois Wesleyan University, Bloomington, IL 61701, USA. [email protected].
  10. South Texas Orthopaedic Research Institute, Laredo, TX 78045, USA. [email protected].
  11. Millennium Pain Center, Bloomington, IL 61704, USA. [email protected].
  12. Department of Psychology, Illinois Wesleyan University, Bloomington, IL 61701, USA. [email protected].
  13. Department of Chemistry, Illinois State University, Normal, IL 61790, USA. [email protected].
  14. Millennium Pain Center, Bloomington, IL 61704, USA. [email protected].
  15. Millennium Pain Center, Bloomington, IL 61704, USA. [email protected].
  16. Geisel School of Medicine, Dartmouth College, Hanover, NH 03755, USA. [email protected].
  17. Millennium Pain Center, Bloomington, IL 61704, USA. [email protected].
  18. Department of Psychology, Illinois Wesleyan University, Bloomington, IL 61701, USA. [email protected].
  19. College of Medicine, Department of Surgery, University of Illinois at Urbana-Champaign, Champaign-Urbana, IL 61801, USA. [email protected].
  20. Millennium Pain Center, Bloomington, IL 61704, USA. [email protected].
  21. Department of Psychology, Illinois Wesleyan University, Bloomington, IL 61701, USA. [email protected].

PMID: 31683631 PMCID: PMC6896182 DOI: 10.3390/brainsci9110303

Abstract

Glial cells comprise the majority of cells in the central nervous system and exhibit diverse functions including the development of persistent neuropathic pain. While earlier theories have proposed that the applied electric field specifically affects neurons, it has been demonstrated that electrical stimulation (ES) of neural tissue modulates gene expression of the glial cells. This study examines the effect of ES on the expression of eight genes related to oxidative stress and neuroprotection in cultured rodent glioma cells. Concentric bipolar electrodes under seven different ES types were used to stimulate cells for 30 min in the presence and absence of extracellular glutamate. ES consisted of rectangular pulses at 50 Hz in varying proportions of anodic and cathodic phases. Real-time reverse-transcribed quantitative polymerase chain reaction was used to determine gene expression using the ∆∆C

Keywords: cell culture; electrical stimulation; gene expression; glial cells; oxidative stress

References

  1. Reg Anesth Pain Med. 2016 Nov/Dec;41(6):750-756 - PubMed
  2. Neurosurgery. 2010 Aug;67(2):367-75 - PubMed
  3. J Trace Elem Med Biol. 2018 Mar;46:62-75 - PubMed
  4. Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10925-30 - PubMed
  5. PLoS One. 2013 Jul 02;8(7):e68312 - PubMed
  6. Neuron Glia Biol. 2007 Nov;3(4):325-34 - PubMed
  7. Neuroscientist. 2007 Feb;13(1):28-37 - PubMed
  8. Neuromodulation. 2016 Aug;19(6):576-86 - PubMed
  9. Pain. 2013 Dec;154 Suppl 1:S10-28 - PubMed
  10. Nucleic Acids Res. 2007 Jul;35(Web Server issue):W43-6 - PubMed
  11. Brain Behav. 2015 Oct 26;5(12):e00414 - PubMed
  12. Nat Commun. 2017 Apr 21;8:15074 - PubMed
  13. J Anat. 2019 Nov;235(5):997-1006 - PubMed
  14. J Neural Eng. 2010 Apr;7(2):26009 - PubMed
  15. Spine (Phila Pa 1976). 2017 Jul 15;42 Suppl 14:S53-S60 - PubMed
  16. Int J Mol Sci. 2016 Sep 06;17(9): - PubMed
  17. Nat Commun. 2012;3:1197 - PubMed
  18. Toxicol In Vitro. 1995 Feb;9(1):39-48 - PubMed
  19. Pain Pract. 2010 May-Jun;10(3):167-84 - PubMed
  20. J Neural Eng. 2011 Aug;8(4):046001 - PubMed
  21. Brain Res Mol Brain Res. 1998 May;56(1-2):108-17 - PubMed
  22. Trends Neurosci. 1996 Aug;19(8):346-52 - PubMed
  23. BMC Bioinformatics. 2012 Jun 18;13:134 - PubMed
  24. Genome Res. 2002 Jun;12(6):996-1006 - PubMed
  25. Mol Pain. 2018 Jan-Dec;14:1744806918817429 - PubMed
  26. J Anat. 2007 Jun;210(6):651-60 - PubMed
  27. Neuroscience. 1996 Jul;73(1):201-8 - PubMed
  28. Free Radic Biol Med. 2011 Sep 1;51(5):951-66 - PubMed
  29. Neuroscience. 2006;137(1):197-209 - PubMed
  30. Nat Rev Neurosci. 2009 Jan;10(1):23-36 - PubMed
  31. Neuroscience. 1981;6(12):2529-37 - PubMed
  32. Science. 1968 Jul 26;161(3839):370-1 - PubMed
  33. Neuroscience. 1992 Jun;48(4):903-14 - PubMed
  34. Am J Physiol. 1997 Nov;273(5):R1771-8 - PubMed
  35. Neuromodulation. 2019 May 9;:null - PubMed
  36. Pain Physician. 2013 Sep-Oct;16(5):E601-13 - PubMed
  37. Cell Death Dis. 2011 Apr 07;2:e141 - PubMed

Publication Types