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Balach MM, Casale CH, Campetelli AN. Erythrocyte plasma membrane potential: past and current methods for its measurement. Biophys Rev. 2019;11(6):995-1005doi: 10.1007/s12551-019-00603-5.
Balach, M. M., Casale, C. H., & Campetelli, A. N. (2019). Erythrocyte plasma membrane potential: past and current methods for its measurement. Biophysical reviews, 11(6), 995-1005. https://doi.org/10.1007/s12551-019-00603-5
Balach, Melisa M, et al. "Erythrocyte plasma membrane potential: past and current methods for its measurement." Biophysical reviews vol. 11,6 (2019): 995-1005. doi: https://doi.org/10.1007/s12551-019-00603-5
Balach MM, Casale CH, Campetelli AN. Erythrocyte plasma membrane potential: past and current methods for its measurement. Biophys Rev. 2019 Dec;11(6):995-1005. doi: 10.1007/s12551-019-00603-5. Epub 2019 Nov 18. PMID: 31741171; PMCID: PMC6874941.
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Biophys Rev. 2019 Dec;11(6):995-1005. doi: 10.1007/s12551-019-00603-5. Epub 2019 Nov 18.

Erythrocyte plasma membrane potential: past and current methods for its measurement.

Biophysical reviews

Melisa M Balach, Cesar H Casale, Alexis N Campetelli

Affiliations

  1. INBIAS-CONICET, Ruta Nacional 36, Km 601, Río Cuarto, Cordoba, Argentina.
  2. Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36, Km 601, Río Cuarto, Cordoba, Argentina.
  3. INBIAS-CONICET, Ruta Nacional 36, Km 601, Río Cuarto, Cordoba, Argentina. [email protected].
  4. Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36, Km 601, Río Cuarto, Cordoba, Argentina. [email protected].

PMID: 31741171 PMCID: PMC6874941 DOI: 10.1007/s12551-019-00603-5

Abstract

The plasma membrane functions both as a natural insulator and a diffusion barrier to the movement of ions. A wide variety of proteins transport and pump ions to generate concentration gradients that result in voltage differences, while ion channels allow ions to move across the membrane down those gradients. Plasma membrane potential is the difference in voltage between the inside and the outside of a biological cell, and it ranges from ~- 3 to ~- 90 mV. Most of the most significant discoveries in this field have been made in excitable cells, such as nerve and muscle cells. Nevertheless, special attention has been paid to some events controlled by changes in membrane potential in non-excitable cells. The origins of several blood disorders, for instance, are related to disturbances at the level of plasma membrane in erythrocytes, the structurally simplest red blood cells. The high simplicity of erythrocytes, in particular, made them perfect candidates for the electrophysiological studies that laid the foundations for understanding the generation, maintenance, and roles of membrane potential. This article summarizes the methodologies that have been used during the past decades to determine Δψ in red blood cells, from seminal microelectrodes, through the use of nuclear magnetic resonance or lipophilic radioactive ions to quantify intra and extracellular ions, to continuously renewed fluorescent potentiometric dyes. We have attempted to highlight the advantages and disadvantages of each methodology, as well as to provide a description of the technical aspects involved.

Keywords: Erythrocytes; Ion homeostasis; Microelectrodes; Plasma membrane potential; Potentiometric dyes

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