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Jiang YK, Xin KY, Ge HW, et al. Upregulation Of Renal GLUT2 And SGLT2 Is Involved In High-Fat Diet-Induced Gestational Diabetes In Mice. Diabetes Metab Syndr Obes. 2019;12:2095-2105doi: 10.2147/DMSO.S221396.
Jiang, Y. K., Xin, K. Y., Ge, H. W., Kong, F. J., & Zhao, G. (2019). Upregulation Of Renal GLUT2 And SGLT2 Is Involved In High-Fat Diet-Induced Gestational Diabetes In Mice. Diabetes, metabolic syndrome and obesity : targets and therapy, 122095-2105. https://doi.org/10.2147/DMSO.S221396
Jiang, Yong-Kuan, et al. "Upregulation Of Renal GLUT2 And SGLT2 Is Involved In High-Fat Diet-Induced Gestational Diabetes In Mice." Diabetes, metabolic syndrome and obesity : targets and therapy vol. 12 (2019): 2095-2105. doi: https://doi.org/10.2147/DMSO.S221396
Jiang YK, Xin KY, Ge HW, Kong FJ, Zhao G. Upregulation Of Renal GLUT2 And SGLT2 Is Involved In High-Fat Diet-Induced Gestational Diabetes In Mice. Diabetes Metab Syndr Obes. 2019 Oct 14;12:2095-2105. doi: 10.2147/DMSO.S221396. eCollection 2019. PMID: 31686881; PMCID: PMC6800457.
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Diabetes Metab Syndr Obes. 2019 Oct 14;12:2095-2105. doi: 10.2147/DMSO.S221396. eCollection 2019.

Upregulation Of Renal GLUT2 And SGLT2 Is Involved In High-Fat Diet-Induced Gestational Diabetes In Mice.

Diabetes, metabolic syndrome and obesity : targets and therapy

Yong-Kuan Jiang, Kai-Yue Xin, Hong-Wei Ge, Fei-Juan Kong, Gang Zhao

Affiliations

  1. Department of Anesthesiology, Ningbo No.6 Hospital, Ningbo, Zhejiang, People's Republic of China.
  2. Department of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China.
  3. Department of Cardiology, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, People's Republic of China.
  4. Department of Urology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, People's Republic of China.
  5. Department of Endocrinology and Metabolism, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
  6. Department of Endocrinology and Metabolism, Xuhui District Central Hospital of Shanghai, Shanghai, People's Republic of China.
  7. Department of Cardiology, Kashgar Prefecture Second People's Hospital, Kashi, Xinjiang, People's Republic of China.

PMID: 31686881 PMCID: PMC6800457 DOI: 10.2147/DMSO.S221396

Abstract

INTRODUCTION: Gestational diabetes mellitus (GDM) is a metabolic disorder during mid- to late-pregnancy characterized by hyperglycemia, insulin resistance and fetal mal-development. Glucose transporter type 2 (GLUT2) and sodium-coupled glucose cotransporters 2 (SGLT2) in the proximal tubules play a critical role in the reabsorption of glucose and have been linked to the occurrence of type 2 diabetes mellitus (T2DM). Our study was designed to investigate the role of GLUT2 and SGLT2 in the pathogenesis of GDM, which is considered a forerunner of T2DM, and investigate the related molecular mechanism.

METHODS: High-fat diet (HFD) was utilized to build a GDM mouse model that closely induces metabolic abnormalities similar to human GDM. Body weight, blood glucose and serum insulin were recorded in the experimental process. Glucose tolerance was determined by the use of an intraperitoneal glucose tolerance test (IPGTT). In addition, levels of GLUT2 and SGLT2 were evaluated to further explore the underlying mechanism of GDM.

RESULTS: HFD feeding induced abnormal glucose metabolism as manifested by increased levels of blood glucose and insulin and prominent glucose intolerance. Additionally, fetal mice from mother feed on HFD showed higher mean body weight. Furthermore, HFD feeding led to an increase in the number of positive cells of GLUT2 and SGLT2 in the renal proximal tubule and the expressions of renal GLUT2 and SGLT2 mRNA and proteins in mice. However, no obvious change was observed in renal morphology.

CONCLUSION: Our study demonstrates a potential involvement of renal GLUT2 and SGLT2 in GDM pathology in an HFD-induced GDM mouse model, which further supports the role of renal GLUT2 and SGLT2 not only in T1DM and T2DM but also in GDM.

© 2019 Jiang et al.

Keywords: GLUT2; SGLT2; gestational diabetes mellitus; insulin resistance; renal threshold for glucose

Conflict of interest statement

The authors declare no conflicts of interest, financial or otherwise in this work.

References

  1. J Membr Biol. 2001 Jul 15;182(2):105-12 - PubMed
  2. Br Med J. 1940 May 11;1(4140):766-8 - PubMed
  3. Scand J Clin Lab Invest. 1958;10(4):364-71 - PubMed
  4. N Engl J Med. 2008 May 8;358(19):1991-2002 - PubMed
  5. Diabetes. 2005 Dec;54(12):3427-34 - PubMed
  6. Metabolism. 2010 Jul;59(7):943-50 - PubMed
  7. Int J Gynaecol Obstet. 2009 Mar;104 Suppl 1:S25-6 - PubMed
  8. Metabolism. 1998 Jun;47(6):663-8 - PubMed
  9. Diabetes. 2004 Dec;53 Suppl 3:S215-9 - PubMed
  10. J Physiol. 2019 Aug;597(16):4175-4192 - PubMed
  11. J Clin Invest. 1951 Feb;30(2):125-9 - PubMed
  12. Pharmacol Rev. 1970 Dec;22(4):485-518 - PubMed
  13. Scand J Clin Lab Invest. 1971 Sep;28(1):101-9 - PubMed
  14. Diabet Med. 2014 Mar;31(3):273-81 - PubMed
  15. CSH Protoc. 2008 May 01;2008:pdb.prot4986 - PubMed
  16. Diabetes Metab Res Rev. 2014 Feb;30(2):140-5 - PubMed
  17. J Pediatr. 2014 Apr;164(4):807-10 - PubMed
  18. Rev Obstet Gynecol. 2008 Summer;1(3):129-34 - PubMed
  19. J Clin Endocrinol Metab. 2016 Jul;101(7):2683-91 - PubMed
  20. J Matern Fetal Neonatal Med. 2012 Jun;25(6):600-10 - PubMed
  21. Endocrinology. 2008 Feb;149(2):717-24 - PubMed
  22. Am J Kidney Dis. 2009 May;53(5):875-83 - PubMed
  23. J Physiol Biochem. 2015 Dec;71(4):649-58 - PubMed
  24. Hum Reprod Update. 2015 Sep-Oct;21(5):575-92 - PubMed
  25. PLoS One. 2015 Feb 27;10(2):e0115992 - PubMed
  26. J Obstet Gynaecol Br Commonw. 1974 Jan;81(1):30-4 - PubMed
  27. Eur J Clin Invest. 2013 Apr;43(4):341-9 - PubMed
  28. Diabet Med. 1998 Jul;15(7):539-53 - PubMed
  29. Mol Cell Endocrinol. 2016 Feb 15;422:192-202 - PubMed
  30. Horm Behav. 2012 Aug;62(3):263-71 - PubMed
  31. Hormones (Athens). 2015 Jul-Sep;14(3):335-44 - PubMed
  32. Psychoneuroendocrinology. 2015 Oct;60:138-50 - PubMed
  33. Am J Obstet Gynecol. 2004 Mar;190(3):858-65 - PubMed
  34. Diabetes Res Clin Pract. 2014 Feb;103(2):176-85 - PubMed
  35. Nutr Metab Cardiovasc Dis. 2016 May;26(5):414-8 - PubMed
  36. Int J Clin Exp Pathol. 2015 May 01;8(5):5196-202 - PubMed
  37. Diabetes Care. 2007 Jul;30 Suppl 2:S112-9 - PubMed
  38. Nephrol Dial Transplant. 2006 Aug;21(8):2166-71 - PubMed

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