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Dassi C, Seyve L, García X, et al. Fibrinography: A Multiwavelength Light-Scattering Assay of Fibrin Structure. Hemasphere. 2019;3(1):e166doi: 10.1097/HS9.0000000000000166.
Dassi, C., Seyve, L., García, X., Bigo, E., Marlu, R., Caton, F., & Polack, B. (2019). Fibrinography: A Multiwavelength Light-Scattering Assay of Fibrin Structure. HemaSphere, 3(1), e166. https://doi.org/10.1097/HS9.0000000000000166
Dassi, Carhel, et al. "Fibrinography: A Multiwavelength Light-Scattering Assay of Fibrin Structure." HemaSphere vol. 3,1 (2019): e166. doi: https://doi.org/10.1097/HS9.0000000000000166
Dassi C, Seyve L, García X, Bigo E, Marlu R, Caton F, Polack B. Fibrinography: A Multiwavelength Light-Scattering Assay of Fibrin Structure. Hemasphere. 2019 Jan 24;3(1):e166. doi: 10.1097/HS9.0000000000000166. eCollection 2019 Feb. PMID: 31723805; PMCID: PMC6745935.
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Hemasphere. 2019 Jan 24;3(1):e166. doi: 10.1097/HS9.0000000000000166. eCollection 2019 Feb.

Fibrinography: A Multiwavelength Light-Scattering Assay of Fibrin Structure.

HemaSphere

Carhel Dassi, Landry Seyve, Xabel García, Emmanuelle Bigo, Raphaël Marlu, François Caton, Benoît Polack

Affiliations

  1. Laboratoire TIMC-IMAG/TheREx, Université Grenoble Alpes, CNRS UMR 5525, Département d'Hématologie, Institut de Biologie et de Pathologie, Centre Hospitalier Universitaire Grenoble-Alpes, Grenoble, France.
  2. Laboratoire Rhéologie et Procédés, Université Grenoble Alpes, CNRS UMR 5520, Grenoble, France.

PMID: 31723805 PMCID: PMC6745935 DOI: 10.1097/HS9.0000000000000166

Abstract

We have previously developed a fibrin structural assay dedicated to purified fibrinogen-thrombin system. Here, we extend the pertinence of this test, called Fibrinography, to tissue factor-triggered plasma coagulation. We show that Fibrinography determines quantitatively the structure of fibrin fibers in plasma with an excellent reproducibility. We compare this assay with the commonly used single wavelength turbidity method, showing that the latter is not a proper structural assay, but determines essentially the fibrinogen content in plasma. In addition, we also show, in model plasmas, that Fibrinography is able to discriminate normal and hypocoagulant plasmas, and even between hypercoagulant plasmas. Therefore, Fibrinography, by measuring the final step of the coagulation cascade, may be used to evaluate patients' plasma in hypo- or hypercoagulant diseases.

Copyright © 2019 the Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the European Hematology Association.

References

  1. Arterioscler Thromb Vasc Biol. 2011 Dec;31(12):e88-99 - PubMed
  2. Thromb Haemost. 2014 Jul 3;112(1):32-42 - PubMed
  3. Blood. 2010 Oct 28;116(17):3341-6 - PubMed
  4. Thromb Haemost. 1993 Oct 18;70(4):617-24 - PubMed
  5. J Thromb Haemost. 2013 Jun;11 Suppl 1:294-305 - PubMed
  6. Blood. 2013 Mar 7;121(10):1712-9 - PubMed
  7. Phys Rev E Stat Nonlin Soft Matter Phys. 2001 Mar;63(3 Pt 1):031401 - PubMed
  8. Blood Coagul Fibrinolysis. 2002 Sep;13(6):533-9 - PubMed
  9. Biophys J. 2000 Jul;79(1):561-83 - PubMed
  10. J Thromb Haemost. 2007 Jul;5 Suppl 1:116-24 - PubMed
  11. J Thromb Haemost. 2008 Feb;6(2):393-5 - PubMed
  12. Eur J Haematol. 2018 Feb;100(2):113-123 - PubMed
  13. Biophys J. 2013 Jun 18;104(12):2774-5 - PubMed
  14. Macromolecules. 1978 Jan-Feb;11(1):46-50 - PubMed
  15. Thromb J. 2015 Jan 23;13(1):4 - PubMed
  16. Biophys J. 2010 May 19;98(10):2281-9 - PubMed
  17. Arterioscler Thromb Vasc Biol. 2012 May;32(5):1320-4 - PubMed
  18. Int J Lab Hematol. 2017 Jun;39(3):301-307 - PubMed
  19. Phys Rev E Stat Nonlin Soft Matter Phys. 2002 Jul;66(1 Pt 1):011913 - PubMed
  20. Biophys J. 2020 Jan 7;118(1):172-181 - PubMed
  21. Clin Chem. 1991 May;37(5):714-9 - PubMed
  22. Thromb Haemost. 2014 Sep 2;112(3):486-94 - PubMed
  23. Thromb J. 2015 Jan 12;13:1 - PubMed
  24. Thromb Haemost. 2002 Jun;87(6):997-1005 - PubMed
  25. Biophys J. 2013 Nov 5;105(9):1967-75 - PubMed
  26. Thromb Haemost. 2014 Nov;112(5):901-8 - PubMed
  27. Arterioscler Thromb Vasc Biol. 2011 Mar;31(3):494-9 - PubMed
  28. Br J Haematol. 2013 Sep;162(6):819-26 - PubMed
  29. Biophys J. 2010 Oct 6;99(7):2018-27 - PubMed
  30. Biochim Biophys Acta. 1989 Jul 27;997(1-2):96-110 - PubMed

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