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Swyers NC, Cody JP, McCaw ME, et al. Telomere-Mediated Chromosomal Truncation for Generating Engineered Minichromosomes in Maize. Curr Protoc Plant Biol. 2016;1(3):488-500doi: 10.1002/cppb.20031.
Swyers, N. C., Cody, J. P., McCaw, M. E., Graham, N. D., Zhao, C., Gaeta, R. T., & Birchler, J. A. (2016). Telomere-Mediated Chromosomal Truncation for Generating Engineered Minichromosomes in Maize. Current protocols in plant biology, 1(3), 488-500. https://doi.org/10.1002/cppb.20031
Swyers, Nathan C, et al. "Telomere-Mediated Chromosomal Truncation for Generating Engineered Minichromosomes in Maize." Current protocols in plant biology vol. 1,3 (2016): 488-500. doi: https://doi.org/10.1002/cppb.20031
Swyers NC, Cody JP, McCaw ME, Graham ND, Zhao C, Gaeta RT, Birchler JA. Telomere-Mediated Chromosomal Truncation for Generating Engineered Minichromosomes in Maize. Curr Protoc Plant Biol. 2016 Sep;1(3):488-500. doi: 10.1002/cppb.20031. PMID: 31725959.
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Curr Protoc Plant Biol. 2016 Sep;1(3):488-500. doi: 10.1002/cppb.20031.

Telomere-Mediated Chromosomal Truncation for Generating Engineered Minichromosomes in Maize.

Current protocols in plant biology

Nathan C Swyers, Jon P Cody, Morgan E McCaw, Nathaniel D Graham, Changzeng Zhao, Robert T Gaeta, James A Birchler

Affiliations

  1. Division of Biological Sciences, University of Missouri, Columbia, Missouri.

PMID: 31725959 DOI: 10.1002/cppb.20031

Abstract

Minichromosomes have been generated in maize using telomere-mediated truncation. Telomere DNA, because of its repetitive nature, can be difficult to manipulate. The protocols in this unit describe two methods for generating the telomere DNA required for the initiation of telomere-mediated truncation. The resulting DNA can then be used with truncation cassettes for introduction into maize via transformation. © 2016 by John Wiley & Sons, Inc.

Copyright © 2016 John Wiley & Sons, Inc.

Keywords: minichromosomes; telomere cloning; telomere-mediated truncation

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