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Narrowe AB, Borton MA, Hoyt DW, et al. Uncovering the Diversity and Activity of Methylotrophic Methanogens in Freshwater Wetland Soils. mSystems. 2019;4(6)doi: 10.1128/mSystems.00320-19.
Narrowe, A. B., Borton, M. A., Hoyt, D. W., Smith, G. J., Daly, R. A., Angle, J. C., Eder, E. K., Wong, A. R., Wolfe, R. A., Pappas, A., Bohrer, G., Miller, C. S., & Wrighton, K. C. (2019). Uncovering the Diversity and Activity of Methylotrophic Methanogens in Freshwater Wetland Soils. mSystems, 4(6), . https://doi.org/10.1128/mSystems.00320-19
Narrowe, Adrienne B, et al. "Uncovering the Diversity and Activity of Methylotrophic Methanogens in Freshwater Wetland Soils." mSystems vol. 4,6 (2019). doi: https://doi.org/10.1128/mSystems.00320-19
Narrowe AB, Borton MA, Hoyt DW, Smith GJ, Daly RA, Angle JC, Eder EK, Wong AR, Wolfe RA, Pappas A, Bohrer G, Miller CS, Wrighton KC. Uncovering the Diversity and Activity of Methylotrophic Methanogens in Freshwater Wetland Soils. mSystems. 2019 Dec 03;4(6). doi: 10.1128/mSystems.00320-19. PMID: 31796563; PMCID: PMC6890927.
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mSystems. 2019 Dec 03;4(6). doi: 10.1128/mSystems.00320-19.

Uncovering the Diversity and Activity of Methylotrophic Methanogens in Freshwater Wetland Soils.

mSystems

Adrienne B Narrowe, Mikayla A Borton, David W Hoyt, Garrett J Smith, Rebecca A Daly, Jordan C Angle, Elizabeth K Eder, Allison R Wong, Richard A Wolfe, Alexandra Pappas, Gil Bohrer, Christopher S Miller, Kelly C Wrighton

Affiliations

  1. Department of Soil and Crop Sciences, Colorado State University, Fort Collins, Colorado, USA.
  2. Pacific Northwest National Laboratory, Richland, Washington, USA.
  3. Department of Microbiology, The Ohio State University, Columbus, Ohio, USA.
  4. Department of Civil, Environmental & Geodetic Engineering, The Ohio State University, Columbus, Ohio, USA.
  5. Department of Integrative Biology, University of Colorado Denver, Denver, Colorado, USA.
  6. Department of Soil and Crop Sciences, Colorado State University, Fort Collins, Colorado, USA [email protected].

PMID: 31796563 PMCID: PMC6890927 DOI: 10.1128/mSystems.00320-19

Abstract

Wetland soils are one of the largest natural contributors to the emission of methane, a potent greenhouse gas. Currently, microbial contributions to methane emissions from these systems emphasize the roles of acetoclastic and hydrogenotrophic methanogens, while less frequently considering methyl-group substrates (e.g., methanol and methylamines). Here, we integrated laboratory and field experiments to explore the potential for methylotrophic methanogenesis in Old Woman Creek (OWC), a temperate freshwater wetland located in Ohio, USA. We first demonstrated the capacity for methylotrophic methanogenesis in these soils using laboratory soil microcosms amended with trimethylamine. However, subsequent field porewater nuclear magnetic resonance (NMR) analyses to identify methanogenic substrates failed to detect evidence for methylamine compounds in soil porewaters, instead noting the presence of the methylotrophic substrate methanol. Accordingly, our wetland soil-derived metatranscriptomic data indicated that methanol utilization by the

Copyright © 2019 Narrowe et al.

Keywords: Methanomassiliicoccales ; metagenomics; metatranscriptomics; methanol; trimethylamine; wetlands

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