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Reinbold R, John T, Spingardi P, et al. Formaldehyde quantification using ampicillin is not selective. Sci Rep. 2019;9(1):18289doi: 10.1038/s41598-019-54610-3.
Reinbold, R., John, T., Spingardi, P., Kawamura, A., Thompson, A. L., Schofield, C. J., & Hopkinson, R. J. (2019). Formaldehyde quantification using ampicillin is not selective. Scientific reports, 9(1), 18289. https://doi.org/10.1038/s41598-019-54610-3
Reinbold, Raphael, et al. "Formaldehyde quantification using ampicillin is not selective." Scientific reports vol. 9,1 (2019): 18289. doi: https://doi.org/10.1038/s41598-019-54610-3
Reinbold R, John T, Spingardi P, Kawamura A, Thompson AL, Schofield CJ, Hopkinson RJ. Formaldehyde quantification using ampicillin is not selective. Sci Rep. 2019 Dec 04;9(1):18289. doi: 10.1038/s41598-019-54610-3. PMID: 31797955; PMCID: PMC6892939.
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Sci Rep. 2019 Dec 04;9(1):18289. doi: 10.1038/s41598-019-54610-3.

Formaldehyde quantification using ampicillin is not selective.

Scientific reports

Raphael Reinbold, Tobias John, Paolo Spingardi, Akane Kawamura, Amber L Thompson, Christopher J Schofield, Richard J Hopkinson

Affiliations

  1. Chemistry Research Laboratory, 12 Mansfield Road, Oxford, OX1 3TA, United Kingdom.
  2. Ludwig Institute for Cancer Research, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7DQ, United Kingdom.
  3. Division of Cardiovascular Medicine, Radcliffe Department of Medicine, Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford, OX3 7BN, United Kingdom.
  4. Chemical Crystallography, Chemistry Research Laboratory, 12 Mansfield Road, Oxford, OX1 3TA, United Kingdom.
  5. Chemistry Research Laboratory, 12 Mansfield Road, Oxford, OX1 3TA, United Kingdom. [email protected].
  6. Chemistry Research Laboratory, 12 Mansfield Road, Oxford, OX1 3TA, United Kingdom. [email protected].
  7. Leicester Institute of Structural and Chemical Biology and School of Chemistry, University of Leicester, Henry Wellcome Building, Lancaster Road, Leicester, LE1 7RH, United Kingdom. [email protected].

PMID: 31797955 PMCID: PMC6892939 DOI: 10.1038/s41598-019-54610-3

Abstract

Formaldehyde (HCHO) is a simple and highly reactive human metabolite but its biochemistry is poorly defined. A limiting factor in HCHO research is lack of validated quantification methods for HCHO relevant to biological samples. We describe spectroscopic studies on a reported fluorescence-based HCHO detection method involving its reaction with ampicillin. The results validate the structure and fluorescence properties of the HCHO-ampicillin reaction product. However, the same adduct is observed after reaction of ampicillin with glyoxylate. Related fluorophores were formed with other biologically relevant carbonyl compounds. Overall, our studies suggest the ampicillin method is not reliable for selective detection and quantification of HCHO in biological samples.

References

  1. J Chromatogr B Biomed Sci Appl. 1997 Jul 4;694(2):401-7 - PubMed
  2. J Chromatogr B Biomed Sci Appl. 2001 Apr 5;753(2):253-7 - PubMed
  3. J Biosci. 2009 Dec;34(6):853-72 - PubMed
  4. Chem Biol. 2010 Sep 24;17(9):925-30 - PubMed
  5. Biomed Chromatogr. 1999 Dec;13(8):513-5 - PubMed
  6. Annu Rev Nutr. 2010 Aug 21;30:57-81 - PubMed
  7. Chemotherapy. 1972;17(3):145-60 - PubMed
  8. Org Biomol Chem. 2010 Nov 7;8(21):4915-20 - PubMed
  9. Age (Dordr). 2013 Jun;35(3):583-96 - PubMed
  10. Chem Sci. 2017 May 1;8(5):4073-4081 - PubMed
  11. Biochemistry. 1975 Mar 25;14(6):1281-96 - PubMed
  12. Blood. 2016 Dec 15;128(24):2774-2784 - PubMed
  13. Drugs. 1989 Apr;37(4):491-522 - PubMed
  14. Neurobiol Aging. 2011 Jan;32(1):31-41 - PubMed
  15. Org Biomol Chem. 2018 May 30;16(21):4021-4032 - PubMed
  16. Biochemistry. 1975 Mar 25;14(6):1297-303 - PubMed
  17. Biochim Biophys Acta. 2014 Dec;1839(12):1416-32 - PubMed
  18. Toxicol Pathol. 2013 Feb;41(2):181-9 - PubMed
  19. Nature. 2017 Aug 31;548(7669):549-554 - PubMed

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