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Ray HA, Stuhl CJ, Gillett-Kaufman JL. Rapid Collection of Floral Fragrance Volatiles using a Headspace Volatile Collection Technique for GC-MS Thermal Desorption Sampling. J Vis Exp. 2019;(154)doi: 10.3791/58928.
Ray, H. A., Stuhl, C. J., & Gillett-Kaufman, J. L. (2019). Rapid Collection of Floral Fragrance Volatiles using a Headspace Volatile Collection Technique for GC-MS Thermal Desorption Sampling. Journal of visualized experiments : JoVE, (154), . https://doi.org/10.3791/58928
Ray, Haleigh A, et al. "Rapid Collection of Floral Fragrance Volatiles using a Headspace Volatile Collection Technique for GC-MS Thermal Desorption Sampling." Journal of visualized experiments : JoVE vol. ,154 (2019). doi: https://doi.org/10.3791/58928
Ray HA, Stuhl CJ, Gillett-Kaufman JL. Rapid Collection of Floral Fragrance Volatiles using a Headspace Volatile Collection Technique for GC-MS Thermal Desorption Sampling. J Vis Exp. 2019 Dec 10;(154). doi: 10.3791/58928. PMID: 31885370.
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J Vis Exp. 2019 Dec 10;(154). doi: 10.3791/58928.

Rapid Collection of Floral Fragrance Volatiles using a Headspace Volatile Collection Technique for GC-MS Thermal Desorption Sampling.

Journal of visualized experiments : JoVE

Haleigh A Ray, Charles J Stuhl, Jennifer L Gillett-Kaufman

Affiliations

  1. Entomology and Nematology Department, University of Florida; [email protected].
  2. Center for Medical, Agricultural and Veterinary Entomology, Agricultural Research Service U.S. Department of Agriculture.
  3. Entomology and Nematology Department, University of Florida.

PMID: 31885370 DOI: 10.3791/58928

Abstract

Fragrances of many flower families have been sampled and the volatiles analyzed. Knowing the compounds that make up the fragrances can be an important step to conservation of flowers that are threatened or endangered. Because floral fragrance is critical for attracting pollinators, this method could be used to better understand or even enhance pollination. We present a protocol using a portable charcoal air filter and vacuum to collect floral fragrance volatiles, which are then analyzed by a GC-MS. By using this method, fragrance volatiles can be sampled using a non-destructive method with a machine that is easily transported. This methodology uses a rapid sampling procedure, cutting sampling time down from 2-3 hours to approximately 10 minutes. Using GC-MS, the fragrance compounds can be identified individually, based on authentic standards. The steps used for collecting fragrance and control data are presented, from material setup to collecting the data output.

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