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Singh N, Prasad P, Das B, et al. Does tumour necrosis factor alpha-induced cyclooxygenase-2 expression lead to spontaneous abortion in Chlamydia trachomatis-infected women. Microb Pathog. 2020;142:103994doi: 10.1016/j.micpath.2020.103994.
Singh, N., Prasad, P., Das, B., & Rastogi, S. (2020). Does tumour necrosis factor alpha-induced cyclooxygenase-2 expression lead to spontaneous abortion in Chlamydia trachomatis-infected women. Microbial pathogenesis, 142103994. https://doi.org/10.1016/j.micpath.2020.103994
Singh, Namita, et al. "Does tumour necrosis factor alpha-induced cyclooxygenase-2 expression lead to spontaneous abortion in Chlamydia trachomatis-infected women." Microbial pathogenesis vol. 142 (2020): 103994. doi: https://doi.org/10.1016/j.micpath.2020.103994
Singh N, Prasad P, Das B, Rastogi S. Does tumour necrosis factor alpha-induced cyclooxygenase-2 expression lead to spontaneous abortion in Chlamydia trachomatis-infected women. Microb Pathog. 2020 Jan 24;142:103994. doi: 10.1016/j.micpath.2020.103994. Epub 2020 Jan 24. PMID: 31988007.
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Microb Pathog. 2020 Jan 24;142:103994. doi: 10.1016/j.micpath.2020.103994. Epub 2020 Jan 24.

Does tumour necrosis factor alpha-induced cyclooxygenase-2 expression lead to spontaneous abortion in Chlamydia trachomatis-infected women.

Microbial pathogenesis

Namita Singh, Priya Prasad, Banashree Das, Sangita Rastogi

Affiliations

  1. Microbiology Laboratory, National Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi, 110 029, India.
  2. Department of Obstetrics & Gynecology, Vardhaman Mahavir Medical College (VMMC) & Safdarjung Hospital, New Delhi, 110 029, India.
  3. Microbiology Laboratory, National Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi, 110 029, India. Electronic address: [email protected].

PMID: 31988007 DOI: 10.1016/j.micpath.2020.103994

Abstract

Cytokines might be involved in spontaneous abortion by triggering inflammatory mediators (Cyclooxygenases (Cox)) leading to spontaneous abortion in Chlamydia trachomatis (Ct)-infected women. Study aimed to quantitate the expression of pro-inflammatory cytokines (TNF-α, IFN-γ, IL-8) and Cox-2 in endometrial curettage tissue (ECT) of Spontaneous Aborters (SA). SA (n = 135) and 120 age-matched controls were enrolled from SJH, New Delhi, India. PCR was performed for detection of Ct MOMP gene (537 bp) in ECT. mRNA expression of pro-inflammatory cytokines and Cox-2 was assessed by real-time qPCR. Data was statistically evaluated. 14.8% SA were diagnosed as Ct-positive. Elevated expression of TNF-α, IFN-γ, IL-8 and Cox-2 was observed in Ct-infected SA. Ct-positive recurrent aborters showed significantly higher cytokine expression. Significant positive correlation was found between expression of Cox-2 and TNF-α in infected SA. Data suggested an increased expression of Th-1 cytokines, particularly TNF-α that induced Cox-2 expression in ECT, leading to spontaneous abortion in Ct-infected pregnant women.

Copyright © 2020. Published by Elsevier Ltd.

Keywords: Chlamydia trachomatis; Cyclooxygenase-2; Pro-inflammatory cytokines; Real time PCR; Spontaneous abortion

Conflict of interest statement

Declaration of competing interest The authors declare no conflicts of interest.

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