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Chen L, Gu L, Geng X, et al. A novel cis antisense RNA AsfD promotes Salmonella enterica serovar Typhi motility and biofilm formation. Microb Pathog. 2020;142:104044doi: 10.1016/j.micpath.2020.104044.
Chen, L., Gu, L., Geng, X., Xu, G., Huang, X., & Zhu, X. (2020). A novel cis antisense RNA AsfD promotes Salmonella enterica serovar Typhi motility and biofilm formation. Microbial pathogenesis, 142104044. https://doi.org/10.1016/j.micpath.2020.104044
Chen, Long, et al. "A novel cis antisense RNA AsfD promotes Salmonella enterica serovar Typhi motility and biofilm formation." Microbial pathogenesis vol. 142 (2020): 104044. doi: https://doi.org/10.1016/j.micpath.2020.104044
Chen L, Gu L, Geng X, Xu G, Huang X, Zhu X. A novel cis antisense RNA AsfD promotes Salmonella enterica serovar Typhi motility and biofilm formation. Microb Pathog. 2020 Feb 04;142:104044. doi: 10.1016/j.micpath.2020.104044. Epub 2020 Feb 04. PMID: 32032766.
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Microb Pathog. 2020 Feb 04;142:104044. doi: 10.1016/j.micpath.2020.104044. Epub 2020 Feb 04.

A novel cis antisense RNA AsfD promotes Salmonella enterica serovar Typhi motility and biofilm formation.

Microbial pathogenesis

Long Chen, Liping Gu, Xinfeng Geng, Guoxin Xu, Xinxiang Huang, Xiaojue Zhu

Affiliations

  1. Department of Clinical Laboratory, The Affiliated Zhangjiagang Hospital of Soochow University, Zhangjiagang, 215600, China.
  2. Department of Biochemistry and Molecular Biology, School of Medicine, Jiangsu University, Zhenjiang, China.
  3. Department of Clinical Laboratory, The Affiliated Zhangjiagang Hospital of Soochow University, Zhangjiagang, 215600, China. Electronic address: [email protected].

PMID: 32032766 DOI: 10.1016/j.micpath.2020.104044

Abstract

Bacterial non-coding RNAs (ncRNAs) can participate in multiple biological processes, including motility, biofilm formation, and virulence. Using high-throughput sequencing and transcriptome analysis of Salmonella enterica serovar Typhi (S. Typhi), we identified a novel antisense RNA located at the opposite strand of the flhDC operon. In this study, a northern blot and qRT-PCR were used to confirm the expression of this newfound antisense RNA in S. Typhi. Moreover, 5' RACE and 3' RT-PCR were performed to reveal the molecular characteristics of the antisense RNA, which was 2079 nt - 2179 nt in length, covered the entire flhDC operon sequence, and termed AsfD. The level of AsfD expression was higher during the stationary phase of S. Typhi and activated by the regulators, OmpR and Fis. When AsfD was overexpressed, the level of flagellar gene flhDC transcription increased; moreover, the level of fliA and fljB expression, as well as the motility and biofilm formation of S. Typhi were also enhanced. The results of this study suggest that AsfD is likely to enhance the motility and biofilm formation of S. Typhi by up-regulating flhDC expression.

Copyright © 2020 Elsevier Ltd. All rights reserved.

Keywords: AsfD; Biofilm; Motility; Non-coding RNA; Salmonella enterica serovar Typhi; flhDC

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