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Front Microbiol. 2020 Jan 31;10:2961. doi: 10.3389/fmicb.2019.02961. eCollection 2019.

Core/Whole Genome Multilocus Sequence Typing and Core Genome SNP-Based Typing of OXA-48-Producing .

Frontiers in microbiology

Elisenda Miro, John W A Rossen, Monika A Chlebowicz, Dag Harmsen, Sylvain Brisse, Virginie Passet, Ferran Navarro, Alex W Friedrich, S García-Cobos

Affiliations

  1. Department of Microbiology, Hospital de la Santa Creu i Sant Pau, Institut d'Investigació Biomèdica Sant Pau (IIB Sant Pau), Barcelona, Spain.
  2. Department of Medical Microbiology and Infection Prevention, University Medical Center Groningen, University of Groningen, Groningen, Netherlands.
  3. ESCMID Study Group for Genomic and Molecular Diagnostics (ESGMD), Basel, Switzerland.
  4. Department of Periodontology and Restorative Dentistry, University of Münster, Münster, Germany.
  5. Biodiversity and Epidemiology of Bacterial Pathogens, Institut Pasteur, Paris, France.

PMID: 32082262 PMCID: PMC7005014 DOI: 10.3389/fmicb.2019.02961

Abstract

Whole-genome sequencing (WGS)-based typing methods have emerged as promising and highly discriminative epidemiological tools. In this study, we combined gene-by-gene allele calling and core genome single nucleotide polymorphism (cgSNP) approaches to investigate the genetic relatedness of a well-characterized collection of OXA-48

Copyright © 2020 Miro, Rossen, Chlebowicz, Harmsen, Brisse, Passet, Navarro, Friedrich and García-Cobos.

Keywords: K. pneumoniae; OXA-48; WGS; cgMLST; molecular epidemiology; wgMLST

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