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Sun DL, Gong ZH, Shao SL, et al. virB11 gene potentially involves in ATP metabolism to provide energy in H. pylori infection. Microb Pathog. 2020;142:104067doi: 10.1016/j.micpath.2020.104067.
Sun, D. L., Gong, Z. H., Shao, S. L., Shi, X. L., Yuan, X. Y., Luo, H., & Wang, M. Y. (2020). virB11 gene potentially involves in ATP metabolism to provide energy in H. pylori infection. Microbial pathogenesis, 142104067. https://doi.org/10.1016/j.micpath.2020.104067
Sun, Da-Lin, et al. "virB11 gene potentially involves in ATP metabolism to provide energy in H. pylori infection." Microbial pathogenesis vol. 142 (2020): 104067. doi: https://doi.org/10.1016/j.micpath.2020.104067
Sun DL, Gong ZH, Shao SL, Shi XL, Yuan XY, Luo H, Wang MY. virB11 gene potentially involves in ATP metabolism to provide energy in H. pylori infection. Microb Pathog. 2020 Feb 13;142:104067. doi: 10.1016/j.micpath.2020.104067. Epub 2020 Feb 13. PMID: 32061915.
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Microb Pathog. 2020 Feb 13;142:104067. doi: 10.1016/j.micpath.2020.104067. Epub 2020 Feb 13.

virB11 gene potentially involves in ATP metabolism to provide energy in H. pylori infection.

Microbial pathogenesis

Da-Lin Sun, Zhen-Hua Gong, Shu-Li Shao, Xiao-Lin Shi, Xiao-Yan Yuan, Hong Luo, Ming-Yi Wang

Affiliations

  1. Department of Central Lab, Weihai Municipal Hospital Affiliated to Dalian Medical University, Weihai, Shandong, 264200, PR China; School of Laboratory Medicine, Dalian Medical University, Dalian, Liaoning, 116044, PR China.
  2. Department of Genetics Lab, Zibo Maternal and Child Health Hospital, Zibo, Shandong, 255000, PR China.
  3. Department of Central Lab, Weihai Municipal Hospital Affiliated to Dalian Medical University, Weihai, Shandong, 264200, PR China.
  4. School of Laboratory Medicine, Dalian Medical University, Dalian, Liaoning, 116044, PR China.
  5. School of Laboratory Medicine, Dalian Medical University, Dalian, Liaoning, 116044, PR China. Electronic address: [email protected].
  6. Department of Central Lab, Weihai Municipal Hospital Affiliated to Dalian Medical University, Weihai, Shandong, 264200, PR China. Electronic address: [email protected].

PMID: 32061915 DOI: 10.1016/j.micpath.2020.104067

Abstract

Helicobater pylori (H. pylori) is the most important bacteria known to be associated with various gastroduodenal diseases. virB11 gene is a structural gene of tfs3a genes cluster in the plasticity region of H. pylori. In this study, the structure and biology of virB11 gene were analyzed and elucidated with bioinformatics analysis. After cloning, expression and purification, VirB11 protein was generated for the cytotoxicity to GES-1 cells and the anti-VirB11 protein antibody production for localization and interaction proteins analysis. The results showed that VirB11 protein is a hydrophilic protein, mainly locates in cell membrane. IL-8 productions from GES-1 cells co-culture with VirB11 protein were increased gradually with time (p < 0.001). The interaction proteins of VirB11 protein were F0F1 ATP synthase subunit alpha, ATP synthase subunit beta and isocitrate dehydrogenase. We demonstrate that VirB11 protein possesses cytotoxicity and potentially plays important roles in ATP metabolism to provide energy in the course of H. pylori infection.

Copyright © 2020 Elsevier Ltd. All rights reserved.

Keywords: Helicobacter pylori; Plasticity region; T4SS; Virulence factor; virB11

Conflict of interest statement

Declaration of competing interest The authors declare that they have no conflicts of interest concerning this article.

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