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Fan HN, Zhu P, Lu YM, et al. Mild changes in the mucosal microbiome during terminal ileum inflammation. Microb Pathog. 2020;142:104104doi: 10.1016/j.micpath.2020.104104.
Fan, H. N., Zhu, P., Lu, Y. M., Guo, J. H., Zhang, J., Qu, G. Q., & Zhu, J. S. (2020). Mild changes in the mucosal microbiome during terminal ileum inflammation. Microbial pathogenesis, 142104104. https://doi.org/10.1016/j.micpath.2020.104104
Fan, Hui-Ning, et al. "Mild changes in the mucosal microbiome during terminal ileum inflammation." Microbial pathogenesis vol. 142 (2020): 104104. doi: https://doi.org/10.1016/j.micpath.2020.104104
Fan HN, Zhu P, Lu YM, Guo JH, Zhang J, Qu GQ, Zhu JS. Mild changes in the mucosal microbiome during terminal ileum inflammation. Microb Pathog. 2020 Feb 28;142:104104. doi: 10.1016/j.micpath.2020.104104. Epub 2020 Feb 28. PMID: 32120004.
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Microb Pathog. 2020 Feb 28;142:104104. doi: 10.1016/j.micpath.2020.104104. Epub 2020 Feb 28.

Mild changes in the mucosal microbiome during terminal ileum inflammation.

Microbial pathogenesis

Hui-Ning Fan, Pei Zhu, Yun-Min Lu, Jing-Hui Guo, Jing Zhang, Guo-Qiang Qu, Jin-Shui Zhu

Affiliations

  1. Department of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.
  2. Department of Gastroenterology, Shanghai University of Medicine and Health Sciences Affiliated Sixth People's Hospital East Campus, Shanghai, 201306, China.
  3. Department of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China. Electronic address: [email protected].

PMID: 32120004 DOI: 10.1016/j.micpath.2020.104104

Abstract

Patients with inflammation in the terminal ileum have high morbidity. In genetically susceptible hosts, chronic intestinal inflammation targeting the resident intestinal microbiota develops, but the microbial signature of the terminal ileum is poorly studied. To improve understanding of the mechanisms underlying the high prevalence of terminal ileum inflammation, we used 16S rRNA sequencing to analyse the mucosa-associated microbiota of the terminal ileum under intestinal homeostasis and inflammation conditions. Mucosal biopsy is the most commonly used sampling technique for assessing microbial communities associated with the intestinal mucosa. Thirty patients (15 with terminal ileum inflammation and 15 controls) underwent colonoscopy and biopsies were taken from the terminal ileum. Diagnosis depended on a combination of endoscopic and histological factors. To determine the composition and diversity of the microbiota, the 16S rRNA was analysed, and a variety of bioinformatics analyses were performed. Among the patients, composition analysis showed that the most abundant phyla identified in the terminal ileum samples were Fusobacteria, Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria. At the phylum level, the relative proportion of Bacteroidetes was lower in patients with inflammation than in control patients. In addition, there was an increase in the abundance of the phyla Proteobacteria and Lentisphaerae in patients with inflammation. The abundances of the dominant microbes in the terminal ileum were not significantly different between patients in an inflammatory state and controls. These results confirm that partial dysbiosis of the intestinal mucosa-associated microbiota composition is associated with terminal ileum inflammation.

Copyright © 2020 Elsevier Ltd. All rights reserved.

Keywords: Biopsies; Inflammation; Microbiota; Terminal ileum

Conflict of interest statement

Declaration of competing interest The authors declare that they have no competing interests.

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