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Ponomareva T, Sliadovskii D, Timchenko M, et al. The effect of hepatopancreas homogenate of the Red king crab on HA-based filler. PeerJ. 2020;8:e8579doi: 10.7717/peerj.8579.
Ponomareva, T., Sliadovskii, D., Timchenko, M., Molchanov, M., Timchenko, A., & Sogorin, E. (2020). The effect of hepatopancreas homogenate of the Red king crab on HA-based filler. PeerJ, 8e8579. https://doi.org/10.7717/peerj.8579
Ponomareva, Tatyana, et al. "The effect of hepatopancreas homogenate of the Red king crab on HA-based filler." PeerJ vol. 8 (2020): e8579. doi: https://doi.org/10.7717/peerj.8579
Ponomareva T, Sliadovskii D, Timchenko M, Molchanov M, Timchenko A, Sogorin E. The effect of hepatopancreas homogenate of the Red king crab on HA-based filler. PeerJ. 2020 Feb 12;8:e8579. doi: 10.7717/peerj.8579. eCollection 2020. PMID: 32095375; PMCID: PMC7023832.
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PeerJ. 2020 Feb 12;8:e8579. doi: 10.7717/peerj.8579. eCollection 2020.

The effect of hepatopancreas homogenate of the Red king crab on HA-based filler.

PeerJ

Tatyana Ponomareva, Dmitrii Sliadovskii, Maria Timchenko, Maxim Molchanov, Alexander Timchenko, Evgeny Sogorin

Affiliations

  1. Federal Research Center "Pushchino Scientific Center for Biological Research of the RAS", Pushchino, Russia.
  2. Institute of Theoretical and Experimental Biophysics of the RAS, Pushchino, Russia.
  3. Institute of Protein Research of the RAS, Pushchino, Russia.

PMID: 32095375 PMCID: PMC7023832 DOI: 10.7717/peerj.8579

Abstract

In this study, several methods were used to analyze the hydrolysis of hyaluronic acid (HA)-based cosmetic fillers by the hepatopancreas homogenate of the Red king crab. The results show that the homogenate and commercially available hyaluronidases have similar hydrolysis activities on the fillers. Atomic force microscopy images reveal that the HA fillers consist mainly of spherical-like particles, which are converted into filamentous structures as a result of hydrolysis by the Red king crab hepatopancreas homogenate. Turbidimetric analysis of the hydrolysis process shows that HA aggregation with acidic albumin exhibits a bell-shaped dependence on reaction time. Analysis of the hydrolysis process by nuclear magnetic resonance shows that HA degradation lasts several days. The maximum rate of the reaction is detected in the 1st h of incubation. The data confirm that the purified homogenate of the Red king crab hepatopancreas exerts hyaluronidase activity on HA-based cosmetic fillers; therefore, it may be considered as a potential therapeutic agent for treating filler complications.

© 2020 Ponomareva et al.

Keywords: Atomic force microscopy; Hepatopancreas; Hyaluronic acid; Hyaluronidase; Hyaluronidase activity; Nuclear magnetic resonance; Red king crab; Treating filler complications; Turbidimetric method

Conflict of interest statement

The authors declare that they have no competing interests.

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