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Libisch B, Keresztény T, Kerényi Z, et al. Metagenomic Analysis of Acquired Antibiotic Resistance Determinants in the Gut Microbiota of Wild Boars . J Vet Res. 2020;64(1):111-118doi: 10.2478/jvetres-2020-0015.
Libisch, B., Keresztény, T., Kerényi, Z., Kocsis, R., Sipos, R., Papp, P. P., & Olasz, F. (2020). Metagenomic Analysis of Acquired Antibiotic Resistance Determinants in the Gut Microbiota of Wild Boars . Journal of veterinary research, 64(1), 111-118. https://doi.org/10.2478/jvetres-2020-0015
Libisch, Balázs, et al. "Metagenomic Analysis of Acquired Antibiotic Resistance Determinants in the Gut Microbiota of Wild Boars ." Journal of veterinary research vol. 64,1 (2020): 111-118. doi: https://doi.org/10.2478/jvetres-2020-0015
Libisch B, Keresztény T, Kerényi Z, Kocsis R, Sipos R, Papp PP, Olasz F. Metagenomic Analysis of Acquired Antibiotic Resistance Determinants in the Gut Microbiota of Wild Boars . J Vet Res. 2020 Feb 29;64(1):111-118. doi: 10.2478/jvetres-2020-0015. eCollection 2020 Mar. PMID: 32258807; PMCID: PMC7105989.
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J Vet Res. 2020 Feb 29;64(1):111-118. doi: 10.2478/jvetres-2020-0015. eCollection 2020 Mar.

Metagenomic Analysis of Acquired Antibiotic Resistance Determinants in the Gut Microbiota of Wild Boars .

Journal of veterinary research

Balázs Libisch, Tibor Keresztény, Zoltán Kerényi, Róbert Kocsis, Rita Sipos, Péter P Papp, Ferenc Olasz

Affiliations

  1. Laboratory of Microbiology, Agricultural Biotechnology Institute, National Agricultural Research and Innovation Centre (NARIC), 2100 Gödöll?, Hungary.
  2. Hungarian Dairy Research Institute Ltd., 9200 Mosonmagyaróvár, Hungary.
  3. BIOMI Ltd., 2100 Gödöll?, Hungary.

PMID: 32258807 PMCID: PMC7105989 DOI: 10.2478/jvetres-2020-0015

Abstract

INTRODUCTION: Land application of manure that contains antibiotics and resistant bacteria may facilitate the establishment of an environmental reservoir of antibiotic-resistant microbes, promoting their dissemination into agricultural and natural habitats. The main objective of this study was to search for acquired antibiotic resistance determinants in the gut microbiota of wild boar populations living in natural habitats.

MATERIAL AND METHODS: Gastrointestinal samples of free-living wild boars were collected in the Zemplén Mountains in Hungary and were characterised by culture-based, metagenomic, and molecular microbiological methods. Bioinformatic analysis of the faecal microbiome of a hunted wild boar from Japan was used for comparative studies. Also, shotgun metagenomic sequencing data of two untreated sewage wastewater samples from North Pest (Hungary) from 2016 were analysed by bioinformatic methods. Minimum spanning tree diagrams for seven-gene MLST profiles of 104

RESULTS: In the ileum of a diarrhoeic boar, a dominant

CONCLUSION: The gastrointestinal microbiota of the free-living wild boars examined in this study carried acquired antibiotic resistance determinants that are highly prevalent among domestic livestock populations.

© 2020 B. Libisch et al. published by Sciendo.

Keywords: antibiotic resistance; intestinal microbiota; natural habitat; tetracycline; wild boar

Conflict of interest statement

Conflict of Interest Conflict of Interest Statement: The authors declare that there is no conflict of interests regarding the publication of this article.

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