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Cheng Z, Combs M, Zhu Q, et al. Genome-Wide RNAi Screen Identifies Regulators of Cardiomyocyte Necrosis. ACS Pharmacol Transl Sci. 2019;2(5):361-371doi: 10.1021/acsptsci.9b00052.
Cheng, Z., Combs, M., Zhu, Q., Xia, P., Opheim, Z., Parker, J., Mack, C. P., & Taylor, J. M. (2019). Genome-Wide RNAi Screen Identifies Regulators of Cardiomyocyte Necrosis. ACS pharmacology & translational science, 2(5), 361-371. https://doi.org/10.1021/acsptsci.9b00052
Cheng, Zhaokang, et al. "Genome-Wide RNAi Screen Identifies Regulators of Cardiomyocyte Necrosis." ACS pharmacology & translational science vol. 2,5 (2019): 361-371. doi: https://doi.org/10.1021/acsptsci.9b00052
Cheng Z, Combs M, Zhu Q, Xia P, Opheim Z, Parker J, Mack CP, Taylor JM. Genome-Wide RNAi Screen Identifies Regulators of Cardiomyocyte Necrosis. ACS Pharmacol Transl Sci. 2019 Sep 04;2(5):361-371. doi: 10.1021/acsptsci.9b00052. eCollection 2019 Oct 11. PMID: 32259070; PMCID: PMC7088983.
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ACS Pharmacol Transl Sci. 2019 Sep 04;2(5):361-371. doi: 10.1021/acsptsci.9b00052. eCollection 2019 Oct 11.

Genome-Wide RNAi Screen Identifies Regulators of Cardiomyocyte Necrosis.

ACS pharmacology & translational science

Zhaokang Cheng, Matthew Combs, Qiang Zhu, Peng Xia, Zachary Opheim, Joel Parker, Christopher P Mack, Joan M Taylor

Affiliations

  1. Department of Pharmaceutical Sciences, Washington State University, Spokane, Washington 99210, United States.
  2. Department of Pathology, Department of Genetics, Lineberger Cancer Center, and McAllister Heart Institute, University of North Carolina, Chapel Hill, North Carolina 27599, United States.

PMID: 32259070 PMCID: PMC7088983 DOI: 10.1021/acsptsci.9b00052

Abstract

Regulation of cellular death is central to nearly all physiological routines and is dysregulated in virtually all diseases. Cell death occurs by two major processes, necrosis which culminates in a pervasive inflammatory response and apoptosis which is largely immunologically inert. As necrosis has long been considered an accidental, unregulated form of cellular death that occurred in response to a harsh environmental stimulus, it was largely ignored as a clinical target. However, recent elegant studies suggest that certain forms of necrosis can be reprogrammed. However, scant little is known about the molecules and pathways that orchestrate calcium-overload-induced necrosis, a main mediator of ischemia/reperfusion (IR)-induced cardiomyocyte cell death. To rectify this critical gap in our knowledge, we performed a novel genome-wide siRNA screen to identify modulators of calcium-induced necrosis in human muscle cells. Our screen identified multiple molecular circuitries that either enhance or inhibit this process, including lysosomal calcium channel TPCN1, mitophagy mediatorTOMM7, Ran-binding protein RanBP9, Histone deacetylase HDAC2, chemokine CCL11, and the Arp2/3 complex regulator glia maturation factor-γ (GMFG). Notably, a number of druggable enzymes were identified, including the proteasome β5 subunit (encoded by PSMB5 gene), which controls the proteasomal chymotrypsin-like peptidase activity. Such findings open up the possibility for the discovery of pharmacological interventions that could provide therapeutic benefits to patients affected by myriad disorders characterized by excessive (or too little) necrotic cell loss, including but not limited to IR injury in the heart and kidney, chronic neurodegenerative disorders, muscular dystrophies, sepsis, and cancers.

Copyright © 2019 American Chemical Society.

Conflict of interest statement

The authors declare no competing financial interest.

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