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Kopf KW, Harral JW, Staker EA, et al. Optimization of combined measures of airway physiology and cardiovascular hemodynamics in mice. Pulm Circ. 2020;10(1):2045894020912937doi: 10.1177/2045894020912937.
Kopf, K. W., Harral, J. W., Staker, E. A., Summers, M. E., Petrache, I., Kheyfets, V., Irwin, D. C., & Majka, S. M. (2020). Optimization of combined measures of airway physiology and cardiovascular hemodynamics in mice. Pulmonary circulation, 10(1), 2045894020912937. https://doi.org/10.1177/2045894020912937
Kopf, Katrina W, et al. "Optimization of combined measures of airway physiology and cardiovascular hemodynamics in mice." Pulmonary circulation vol. 10,1 (2020): 2045894020912937. doi: https://doi.org/10.1177/2045894020912937
Kopf KW, Harral JW, Staker EA, Summers ME, Petrache I, Kheyfets V, Irwin DC, Majka SM. Optimization of combined measures of airway physiology and cardiovascular hemodynamics in mice. Pulm Circ. 2020 Mar 13;10(1):2045894020912937. doi: 10.1177/2045894020912937. eCollection 2020. PMID: 32206308; PMCID: PMC7074541.
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Pulm Circ. 2020 Mar 13;10(1):2045894020912937. doi: 10.1177/2045894020912937. eCollection 2020.

Optimization of combined measures of airway physiology and cardiovascular hemodynamics in mice.

Pulmonary circulation

Katrina W Kopf, Julie W Harral, Emily A Staker, Megan E Summers, Irina Petrache, Vitaly Kheyfets, David C Irwin, Susan M Majka

Affiliations

  1. Biological Resource Center, National Jewish Health, Denver, USA.
  2. Department of Medicine, Division of Pulmonary, Critical Care & Sleep Medicine, National Jewish Health, Denver, USA.
  3. Department of Bioengineering, Anschutz Medical Campus University of Colorado, Aurora, USA.
  4. Department of Medicine, Division of Cardiology, Anschutz Medical Campus University of Colorado, Aurora, USA.
  5. Department of Biomedical Research, National Jewish Health, Denver, USA.
  6. Gates Center for Regenerative Medicine and Stem Cell Biology and Cardiology University of Colorado Medical Center, Aurora, USA.

PMID: 32206308 PMCID: PMC7074541 DOI: 10.1177/2045894020912937

Abstract

Pulmonary hypertension may arise as a complication of chronic lung disease typically associated with tissue hypoxia, as well as infectious agents or injury eliciting a type 2 immune response. The onset of pulmonary hypertension in this setting (classified as Group 3) often complicates treatment and worsens prognosis of chronic lung disease. Chronic lung diseases such as chronic obstructive lung disease (COPD), emphysema, and interstitial lung fibrosis impair airflow and alter lung elastance in addition to affecting pulmonary vascular hemodynamics that may culminate in right ventricle dysfunction. To date, functional endpoints in murine models of chronic lung disease have typically been limited to separately measuring airway and lung parenchyma physiology. These approaches may be lengthy and require a large number of animals per experiment. Here, we provide a detailed protocol for combined assessment of airway physiology with cardiovascular hemodynamics in mice. Ultimately, a comprehensive overview of pulmonary function in murine models of injury and disease will facilitate the integration of studies of the airway and vascular biology necessary to understand underlying pathophysiology of Group 3 pulmonary hypertension.

© The Author(s) 2020.

Keywords: Group 3 pulmonary hypertension; airway physiology; cardiovascular hemodynamics; vascular remodeling

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