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Manna B, Retna Raj C. Covalent functionalization and electrochemical tuning of reduced graphene oxide for the bioelectrocatalytic sensing of serum lactate. J Mater Chem B. 2016;4(26):4585-4593doi: 10.1039/c6tb00721j.
Manna, B., & Retna Raj, C. (2016). Covalent functionalization and electrochemical tuning of reduced graphene oxide for the bioelectrocatalytic sensing of serum lactate. Journal of materials chemistry. B, 4(26), 4585-4593. https://doi.org/10.1039/c6tb00721j
Manna, Bhaskar, and Retna Raj, C. "Covalent functionalization and electrochemical tuning of reduced graphene oxide for the bioelectrocatalytic sensing of serum lactate." Journal of materials chemistry. B vol. 4,26 (2016): 4585-4593. doi: https://doi.org/10.1039/c6tb00721j
Manna B, Retna Raj C. Covalent functionalization and electrochemical tuning of reduced graphene oxide for the bioelectrocatalytic sensing of serum lactate. J Mater Chem B. 2016 Jul 14;4(26):4585-4593. doi: 10.1039/c6tb00721j. Epub 2016 Jun 16. PMID: 32263401.
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J Mater Chem B. 2016 Jul 14;4(26):4585-4593. doi: 10.1039/c6tb00721j. Epub 2016 Jun 16.

Covalent functionalization and electrochemical tuning of reduced graphene oxide for the bioelectrocatalytic sensing of serum lactate.

Journal of materials chemistry. B

Bhaskar Manna, C Retna Raj

Affiliations

  1. Functional Materials and Electrochemistry Laboratory, Department of Chemistry, Indian Institute of Technology, Kharagpur 721302, West Bengal, India. [email protected].

PMID: 32263401 DOI: 10.1039/c6tb00721j

Abstract

Lactate is a byproduct of glycolysis and serum lactate can be used as a non-invasive biomarker in risk-stratifying patients with several life-threatening diseases. Herein, we describe the bioelectrocatalytic sensing of lactate using a covalently functionalized reduced graphene oxide (rGO)-based material. The development of a lactate biosensor involves the covalent functionalization of rGO with the p-nitrophenyl moiety, electrochemical generation of a surface-confined redox mediator and immobilization of l-lactate dehydrogenase (LDH). The covalently functionalized rGO was characterized by XRD, XPS, FTIR, Raman, resistivity and electrochemical measurements. The covalent attachment of the nitrophenyl moiety on the basal plane of the carbon network significantly influences the capacitive properties of rGO. The chemically functionalized rGO was electrochemically tuned to generate a redox mediator (rGO-PhNHOH). An electrochemically generated redox couple (PhNHOH/PhNO) exhibits reversible voltammetric response at ∼-0.06 V with a surface coverage of (7.19 ± 0.26) × 10

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