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Díaz Lantada A, Mazarío Picazo N, Guttmann M, et al. Soft-Lithography of Polyacrylamide Hydrogels Using Microstructured Templates: Towards Controlled Cell Populations on Biointerfaces. Materials (Basel). 2020;13(7)doi: 10.3390/ma13071586.
Díaz Lantada, A., Mazarío Picazo, N., Guttmann, M., Wissmann, M., Schneider, M., Worgull, M., Hengsbach, S., Rupp, F., Bade, K., & Plaza, G. R. (2020). Soft-Lithography of Polyacrylamide Hydrogels Using Microstructured Templates: Towards Controlled Cell Populations on Biointerfaces. Materials (Basel, Switzerland), 13(7), . https://doi.org/10.3390/ma13071586
Díaz Lantada, Andrés, et al. "Soft-Lithography of Polyacrylamide Hydrogels Using Microstructured Templates: Towards Controlled Cell Populations on Biointerfaces." Materials (Basel, Switzerland) vol. 13,7 (2020). doi: https://doi.org/10.3390/ma13071586
Díaz Lantada A, Mazarío Picazo N, Guttmann M, Wissmann M, Schneider M, Worgull M, Hengsbach S, Rupp F, Bade K, Plaza GR. Soft-Lithography of Polyacrylamide Hydrogels Using Microstructured Templates: Towards Controlled Cell Populations on Biointerfaces. Materials (Basel). 2020 Mar 30;13(7). doi: 10.3390/ma13071586. PMID: 32235578; PMCID: PMC7177395.
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Materials (Basel). 2020 Mar 30;13(7). doi: 10.3390/ma13071586.

Soft-Lithography of Polyacrylamide Hydrogels Using Microstructured Templates: Towards Controlled Cell Populations on Biointerfaces.

Materials (Basel, Switzerland)

Andrés Díaz Lantada, Noelia Mazarío Picazo, Markus Guttmann, Markus Wissmann, Marc Schneider, Matthias Worgull, Stefan Hengsbach, Florian Rupp, Klaus Bade, Gustavo R Plaza

Affiliations

  1. Product Development Laboratory, Mechanical Engineering Department, Universidad Politécnica de Madrid, c/José Gutiérrez Abascal 2, 28006 Madrid, Spain.
  2. Centre for Biomedical Technology, Universidad Politécnica de Madrid, Parque Científico y Tecnológico de la UPM, Crta. M40, km. 38, 28223 Pozuelo de Alarcón, Madrid, Spain.
  3. Institute of Microstructure Technology, Karlsruhe Institute of Technology, Hermann-von-Helmholtz Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.

PMID: 32235578 PMCID: PMC7177395 DOI: 10.3390/ma13071586

Abstract

Polyacrylamide hydrogels are interesting materials for studying cells and cell-material interactions, thanks to the possibility of precisely adjusting their stiffness, shear modulus and porosity during synthesis, and to the feasibility of processing and manufacturing them towards structures and devices with controlled morphology and topography. In this study a novel approach, related to the processing of polyacrylamide hydrogels using soft-lithography and employing microstructured templates, is presented. The main novelty relies on the design and manufacturing processes used for achieving the microstructured templates, which are transferred by soft-lithography, with remarkable level of detail, to the polyacrylamide hydrogels. The conceived process is demonstrated by patterning polyacrylamide substrates with a set of vascular-like and parenchymal-like textures, for controlling cell populations. Final culture of amoeboid cells, whose dynamics is affected by the polyacrylamide patterns, provides a preliminary validation of the described strategy and helps to discuss its potentials.

Keywords: biointerfaces; hot-embossing; polyacrylamide hydrogels; polymer microfabrication; soft-lithography; surface patterning

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