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McCann MR, McHugh CE, Kirby M, et al. A Multivariate Metabolomics Method for Estimating Platelet Mitochondrial Oxygen Consumption Rates in Patients with Sepsis. Metabolites. 2020;10(4)doi: 10.3390/metabo10040139.
McCann, M. R., McHugh, C. E., Kirby, M., Jennaro, T. S., Jones, A. E., Stringer, K. A., & Puskarich, M. A. (2020). A Multivariate Metabolomics Method for Estimating Platelet Mitochondrial Oxygen Consumption Rates in Patients with Sepsis. Metabolites, 10(4), . https://doi.org/10.3390/metabo10040139
McCann, Marc R, et al. "A Multivariate Metabolomics Method for Estimating Platelet Mitochondrial Oxygen Consumption Rates in Patients with Sepsis." Metabolites vol. 10,4 (2020). doi: https://doi.org/10.3390/metabo10040139
McCann MR, McHugh CE, Kirby M, Jennaro TS, Jones AE, Stringer KA, Puskarich MA. A Multivariate Metabolomics Method for Estimating Platelet Mitochondrial Oxygen Consumption Rates in Patients with Sepsis. Metabolites. 2020 Apr 02;10(4). doi: 10.3390/metabo10040139. PMID: 32252461; PMCID: PMC7240966.
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Metabolites. 2020 Apr 02;10(4). doi: 10.3390/metabo10040139.

A Multivariate Metabolomics Method for Estimating Platelet Mitochondrial Oxygen Consumption Rates in Patients with Sepsis.

Metabolites

Marc R McCann, Cora E McHugh, Maggie Kirby, Theodore S Jennaro, Alan E Jones, Kathleen A Stringer, Michael A Puskarich

Affiliations

  1. The NMR Metabolomics Laboratory, Department of Clinical Pharmacy, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA.
  2. Department of Emergency Medicine, University of Mississippi Medical Center, Jackson, MS 39216, USA.
  3. Division of Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine, University of Michigan, Ann Arbor, MI 48109, USA.
  4. Michigan Center for Integrative Research in Critical Care, University of Michigan, Ann Arbor, MI 48109, USA.
  5. Department of Emergency Medicine, University of Minnesota, Minneapolis, MN 55455, USA.

PMID: 32252461 PMCID: PMC7240966 DOI: 10.3390/metabo10040139

Abstract

BACKGROUND: Sepsis-induced alterations in mitochondrial function contribute to organ dysfunction and mortality. Measuring mitochondrial function in vital organs is neither feasible nor practical, highlighting the need for non-invasive approaches. Mitochondrial function may be reflected in the concentrations of metabolites found in platelets and whole blood (WB) samples. We proposed to use these as alternates to indirectly estimate platelet mitochondrial oxygen consumption rate (mOCR) in sepsis patients.

METHODS: We determined the relationships between platelet mOCR and metabolites in both platelets and WB, as measured by quantitative

RESULTS: The differences in adjusted-R

CONCLUSIONS: Our data suggest there are groups of metabolites in WB (leucine, acetylcarnitine) and platelets (creatine, ADP, glucose, taurine) that are associated with platelet mOCR. Thus, WB and platelet metabolites could be used to estimate platelet mOCR.

Keywords: acetylcarnitine; bioenergetics; metabolism; mitochondria; mitochondrial function; mitochondrial respiration; nuclear magnetic resonance spectroscopy

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