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Hoy CFO, Kushiro K, Yamaoka Y, et al. Rapid multiplex microfiber-based immunoassay for anti-MERS-CoV antibody detection. Sens Biosensing Res. 2019;26:100304doi: 10.1016/j.sbsr.2019.100304.
Hoy, C. F. O., Kushiro, K., Yamaoka, Y., Ryo, A., & Takai, M. (2019). Rapid multiplex microfiber-based immunoassay for anti-MERS-CoV antibody detection. Sensing and Bio-Sensing Research, 26100304. https://doi.org/10.1016/j.sbsr.2019.100304
Hoy, Carlton F O, et al. "Rapid multiplex microfiber-based immunoassay for anti-MERS-CoV antibody detection." Sensing and Bio-Sensing Research vol. 26 (2019): 100304. doi: https://doi.org/10.1016/j.sbsr.2019.100304
Hoy CFO, Kushiro K, Yamaoka Y, Ryo A, Takai M. Rapid multiplex microfiber-based immunoassay for anti-MERS-CoV antibody detection. Sens Biosensing Res. 2019 Nov;26:100304. doi: 10.1016/j.sbsr.2019.100304. Epub 2019 Oct 14. PMID: 32289017; PMCID: PMC7104066.
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Sens Biosensing Res. 2019 Nov;26:100304. doi: 10.1016/j.sbsr.2019.100304. Epub 2019 Oct 14.

Rapid multiplex microfiber-based immunoassay for anti-MERS-CoV antibody detection.

Sensing and Bio-Sensing Research

Carlton F O Hoy, Keiichiro Kushiro, Yutaro Yamaoka, Akihide Ryo, Madoka Takai

Affiliations

  1. Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan.
  2. Department of Microbiology, School of Medicine, Yokohama City University, Yokohama, Japan.
  3. Isehara Research Laboratory, Technology and Development Division, Kanto Chemical Co., Inc., Isehara, Japan.

PMID: 32289017 PMCID: PMC7104066 DOI: 10.1016/j.sbsr.2019.100304

Abstract

On-site multiplex biosensors for innate immunity antibodies are ideal tools for monitoring health status of individuals against various diseases. This study introduces a novel antibody immunoassay testing platform incorporating microfiber-based arrays of antigens to capture specific antibodies. The fabrication and setup of the device revolved around electrospun polystyrene (ESPS) microfibers that act as three-dimensional membrane filters, capable of rapid and multifold analyte capture. In particular, the ESPS microfibers were patterned through localized oxygen plasma to create hydrophilic zones that facilitate fluid flows and immobilizations of antigens. The bulk of this robust antibody immunoassay platform could be installed into a compact syringe-driven cassette device, which could perform multiplex antibody immunoassay for antibodies specifically against Middle East respiratory syndrome coronavirus (MERS-CoV) with rapid preparation amounting to a total of 5 min, as well as high sensitivity and specificity for the MERS-CoV down to 200 μg/mL.

© 2019 The Authors.

Keywords: MERS; Microfiber; Multiplex immunoassay; POC; Rapid

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