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Altay G, Tosi S, García-Díaz M, et al. Imaging the Cell Morphological Response to 3D Topography and Curvature in Engineered Intestinal Tissues. Front Bioeng Biotechnol. 2020;8:294doi: 10.3389/fbioe.2020.00294.
Altay, G., Tosi, S., García-Díaz, M., & Martínez, E. (2020). Imaging the Cell Morphological Response to 3D Topography and Curvature in Engineered Intestinal Tissues. Frontiers in bioengineering and biotechnology, 8294. https://doi.org/10.3389/fbioe.2020.00294
Altay, Gizem, et al. "Imaging the Cell Morphological Response to 3D Topography and Curvature in Engineered Intestinal Tissues." Frontiers in bioengineering and biotechnology vol. 8 (2020): 294. doi: https://doi.org/10.3389/fbioe.2020.00294
Altay G, Tosi S, García-Díaz M, Martínez E. Imaging the Cell Morphological Response to 3D Topography and Curvature in Engineered Intestinal Tissues. Front Bioeng Biotechnol. 2020 Apr 07;8:294. doi: 10.3389/fbioe.2020.00294. eCollection 2020. PMID: 32318564; PMCID: PMC7154059.
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Front Bioeng Biotechnol. 2020 Apr 07;8:294. doi: 10.3389/fbioe.2020.00294. eCollection 2020.

Imaging the Cell Morphological Response to 3D Topography and Curvature in Engineered Intestinal Tissues.

Frontiers in bioengineering and biotechnology

Gizem Altay, Sébastien Tosi, María García-Díaz, Elena Martínez

Affiliations

  1. Biomimetic Systems for Cell Engineering, Institute for Bioengineering of Catalonia, The Barcelona Institute of Science and Technology, Barcelona, Spain.
  2. Advanced Digital Microscopy Core Facility, Institute for Research in Biomedicine, The Barcelona Institute of Science and Technology, Barcelona, Spain.
  3. Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina, Madrid, Spain.
  4. Department of Electronics and Biomedical Engineering, University of Barcelona, Barcelona, Spain.

PMID: 32318564 PMCID: PMC7154059 DOI: 10.3389/fbioe.2020.00294

Abstract

While conventional cell culture methodologies have relied on flat, two-dimensional cell monolayers, three-dimensional engineered tissues are becoming increasingly popular. Often, engineered tissues can mimic the complex architecture of native tissues, leading to advancements in reproducing physiological functional properties. In particular, engineered intestinal tissues often use hydrogels to mimic villi structures. These finger-like protrusions of a few hundred microns in height have a well-defined topography and curvature. Here, we examined the cell morphological response to these villus-like microstructures at single-cell resolution using a novel embedding method that allows for the histological processing of these delicate hydrogel structures. We demonstrated that by using photopolymerisable poly(ethylene) glycol as an embedding medium, the villus-like microstructures were successfully preserved after sectioning with vibratome or cryotome. Moreover, high-resolution imaging of these sections revealed that cell morphology, nuclei orientation, and the expression of epithelial polarization markers were spatially encoded along the vertical axis of the villus-like microstructures and that this cell morphological response was dramatically affected by the substrate curvature. These findings, which are in good agreement with the data reported for

Copyright © 2020 Altay, Tosi, García-Díaz and Martínez.

Keywords: cell morphology; cell orientation; confocal microscopy; histological section; hydrogel scaffold; small intestine; substrate curvature; villus

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