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Wang Y, Chen L, Pandak WM, et al. High Glucose Induces Lipid Accumulation via 25-Hydroxycholesterol DNA-CpG Methylation. iScience. 2020;23(5):101102doi: 10.1016/j.isci.2020.101102.
Wang, Y., Chen, L., Pandak, W. M., Heuman, D., Hylemon, P. B., & Ren, S. (2020). High Glucose Induces Lipid Accumulation via 25-Hydroxycholesterol DNA-CpG Methylation. iScience, 23(5), 101102. https://doi.org/10.1016/j.isci.2020.101102
Wang, Yaping, et al. "High Glucose Induces Lipid Accumulation via 25-Hydroxycholesterol DNA-CpG Methylation." iScience vol. 23,5 (2020): 101102. doi: https://doi.org/10.1016/j.isci.2020.101102
Wang Y, Chen L, Pandak WM, Heuman D, Hylemon PB, Ren S. High Glucose Induces Lipid Accumulation via 25-Hydroxycholesterol DNA-CpG Methylation. iScience. 2020 May 22;23(5):101102. doi: 10.1016/j.isci.2020.101102. Epub 2020 Apr 29. PMID: 32408171; PMCID: PMC7225732.
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iScience. 2020 May 22;23(5):101102. doi: 10.1016/j.isci.2020.101102. Epub 2020 Apr 29.

High Glucose Induces Lipid Accumulation via 25-Hydroxycholesterol DNA-CpG Methylation.

iScience

Yaping Wang, Lanming Chen, William M Pandak, Douglas Heuman, Phillip B Hylemon, Shunlin Ren

Affiliations

  1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Department of Internal Medicine, Virginia Commonwealth University/McGuire VA Medical Centre, Research 151, 1201 Broad Rock Boulevard, Richmond, VA 23249, USA.
  2. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
  3. Department of Internal Medicine, Virginia Commonwealth University/McGuire VA Medical Centre, Research 151, 1201 Broad Rock Boulevard, Richmond, VA 23249, USA.
  4. Department of Internal Medicine, Virginia Commonwealth University/McGuire VA Medical Centre, Research 151, 1201 Broad Rock Boulevard, Richmond, VA 23249, USA. Electronic address: [email protected].

PMID: 32408171 PMCID: PMC7225732 DOI: 10.1016/j.isci.2020.101102

Abstract

This work investigates the relationship between high-glucose (HG) culture, CpG methylation of genes involved in cell signaling pathways, and the regulation of carbohydrate and lipid metabolism in hepatocytes. The results indicate that HG leads to an increase in nuclear 25-hydroxycholesterol (25HC), which specifically activates DNA methyltransferase-1 (DNMT1), and regulates gene expression involved in intracellular lipid metabolism. The results show significant increases in

Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.

Keywords: Biological Sciences; Molecular Genetics; Systems Biology

Conflict of interest statement

Declaration of Interests S.R. and Virginia Commonwealth University obtain license-related payments from DURECT Corporation, Cupertino, CA.

References

  1. J Cardiovasc Pharmacol. 2019 Aug;74(2):152-161 - PubMed
  2. Nature. 1996 Oct 24;383(6602):728-31 - PubMed
  3. Cell Rep. 2019 Jan 8;26(2):346-355.e3 - PubMed
  4. Mol Aspects Med. 2016 Jun;49:8-22 - PubMed
  5. Epigenetics Chromatin. 2019 Oct 8;12(1):59 - PubMed
  6. J Biol Chem. 1998 Dec 18;273(51):34316-27 - PubMed
  7. Nature. 2001 Feb 15;409(6822):860-921 - PubMed
  8. Sci Rep. 2018 Sep 14;8(1):13857 - PubMed
  9. Adv Exp Med Biol. 2017;960:443-467 - PubMed
  10. Genome Res. 2014 Oct;24(10):1613-23 - PubMed
  11. Acta Diabetol. 2015 Jun;52(3):497-503 - PubMed
  12. Mol Pharmacol. 2013 Mar;83(3):648-58 - PubMed
  13. Mol Nutr Food Res. 2016 Apr;60(4):909-21 - PubMed
  14. Diabetes. 2002 Dec;51 Suppl 3:S414-20 - PubMed
  15. Antioxid Redox Signal. 2011 Feb 15;14(4):649-61 - PubMed
  16. Biochem Biophys Res Commun. 2007 Sep 7;360(4):802-8 - PubMed
  17. Handb Exp Pharmacol. 2016;233:51-71 - PubMed
  18. Cell Mol Life Sci. 2008 May;65(10):1509-22 - PubMed
  19. J Gen Virol. 2017 Jun;98(6):1467-1476 - PubMed
  20. Clin Epigenetics. 2018 Apr 25;10:60 - PubMed
  21. Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12027-32 - PubMed
  22. Nat Clin Pract Oncol. 2005 Dec;2 Suppl 1:S4-11 - PubMed
  23. J Pathol. 2018 Nov;246(3):277-288 - PubMed
  24. Lipids. 2010 Sep;45(9):821-32 - PubMed
  25. J Clin Med. 2019 Jan 13;8(1): - PubMed
  26. Proc Natl Acad Sci U S A. 2009 Sep 29;106(39):16764-9 - PubMed
  27. Am J Physiol Endocrinol Metab. 2014 Jan 15;306(2):E123-30 - PubMed
  28. Mol Med Rep. 2018 Sep;18(3):3251-3261 - PubMed
  29. Front Oncol. 2014 May 01;4:80 - PubMed
  30. Cancer Res. 2018 Oct 1;78(19):5548-5560 - PubMed
  31. Biochim Biophys Acta. 2013 Oct;1830(10):4407-16 - PubMed
  32. J Lipid Res. 2007 Dec;48(12):2587-96 - PubMed
  33. Annu Rev Pharmacol Toxicol. 2016;56:447-67 - PubMed
  34. Am J Physiol Endocrinol Metab. 2008 Dec;295(6):E1369-79 - PubMed
  35. J Leukoc Biol. 2010 Dec;88(6):1071-2 - PubMed
  36. Cell Physiol Biochem. 2018;49(2):645-652 - PubMed
  37. Mol Med Rep. 2012 Apr;5(4):895-900 - PubMed
  38. Hum Reprod. 2018 Mar 1;33(3):474-481 - PubMed
  39. J Virol. 2018 Aug 29;92(18): - PubMed
  40. Lipids Health Dis. 2019 Dec 28;18(1):230 - PubMed
  41. Curr Opin Genet Dev. 2010 Apr;20(2):164-70 - PubMed
  42. Immunity. 2017 Mar 21;46(3):446-456 - PubMed
  43. Nat Immunol. 2004 Dec;5(12):1266-74 - PubMed
  44. Proc Natl Acad Sci U S A. 2020 Feb 25;117(8):4328-4336 - PubMed
  45. Nutrients. 2018 Oct 14;10(10): - PubMed
  46. Neuropsychopharmacology. 2013 Jan;38(1):23-38 - PubMed
  47. PLoS One. 2018 Jan 23;13(1):e0191023 - PubMed
  48. BMC Med Genet. 2018 Feb 21;19(1):28 - PubMed
  49. J Virol. 2017 Sep 12;91(19): - PubMed
  50. Oncotarget. 2017 Jan 3;8(1):1845-1859 - PubMed
  51. Cell. 2012 Mar 2;148(5):852-71 - PubMed
  52. Cell. 1992 Jun 12;69(6):905-14 - PubMed
  53. PLoS One. 2012;7(8):e41949 - PubMed
  54. Nat Immunol. 2013 Sep;14(9):893-900 - PubMed
  55. Immunity. 2013 Jan 24;38(1):92-105 - PubMed
  56. IUBMB Life. 2009 Jan;61(1):47-55 - PubMed
  57. Genes Dev. 2000 Nov 15;14(22):2831-8 - PubMed
  58. Diabetologia. 2017 Feb;60(2):215-225 - PubMed
  59. Nat Commun. 2015 May 12;6:7079 - PubMed
  60. Nat Rev Immunol. 2014 Nov;14(11):731-43 - PubMed
  61. Biomolecules. 2017 Feb 14;7(1): - PubMed
  62. Handb Exp Pharmacol. 2016;233:29-49 - PubMed
  63. J Mol Endocrinol. 2017 May;58(4):R225-R240 - PubMed
  64. Mol Biol Rep. 2010 Dec;37(8):3833-9 - PubMed

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