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Dimitrijevic Carlsson A, Ghafouri B, Starkhammar Johansson C, et al. Unstimulated Parotid Saliva Sampling in Juvenile Idiopathic Arthritis and Healthy Controls: A Proof-of-Concept Study on Biomarkers. Diagnostics (Basel). 2020;10(4)doi: 10.3390/diagnostics10040251.
Dimitrijevic Carlsson, A., Ghafouri, B., Starkhammar Johansson, C., & Alstergren, P. (2020). Unstimulated Parotid Saliva Sampling in Juvenile Idiopathic Arthritis and Healthy Controls: A Proof-of-Concept Study on Biomarkers. Diagnostics (Basel, Switzerland), 10(4), . https://doi.org/10.3390/diagnostics10040251
Dimitrijevic Carlsson, Alexandra, et al. "Unstimulated Parotid Saliva Sampling in Juvenile Idiopathic Arthritis and Healthy Controls: A Proof-of-Concept Study on Biomarkers." Diagnostics (Basel, Switzerland) vol. 10,4 (2020). doi: https://doi.org/10.3390/diagnostics10040251
Dimitrijevic Carlsson A, Ghafouri B, Starkhammar Johansson C, Alstergren P. Unstimulated Parotid Saliva Sampling in Juvenile Idiopathic Arthritis and Healthy Controls: A Proof-of-Concept Study on Biomarkers. Diagnostics (Basel). 2020 Apr 24;10(4). doi: 10.3390/diagnostics10040251. PMID: 32344523; PMCID: PMC7236018.
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Diagnostics (Basel). 2020 Apr 24;10(4). doi: 10.3390/diagnostics10040251.

Unstimulated Parotid Saliva Sampling in Juvenile Idiopathic Arthritis and Healthy Controls: A Proof-of-Concept Study on Biomarkers.

Diagnostics (Basel, Switzerland)

Alexandra Dimitrijevic Carlsson, Bijar Ghafouri, Carin Starkhammar Johansson, Per Alstergren

Affiliations

  1. Center For Oral Rehabilitation, Torkelbergsgatan 11, 581 85 Linköping, Department of Biomedical and Clinical Science, Linköping University; postal code 581 83 Linköping, Sweden.
  2. Orofacial Pain Unit, Faculty of Odontology, Malmö University, Carl Gustafs väg 34, 214 21 Malmö, Sweden.
  3. Pain and Rehabilitation Centre, and Department of Health, Medicine and Caring Sciences, 58183 Linköping, Sweden.
  4. Orofacial Pain and Jaw Function, Institute of Dental Medicine, Karolinska Institute, Alfred Nobels allé 8, 141 52 Huddinge, Sweden.
  5. Specialized Pain Rehabilitation, Skåne University Hospital, Lasarettsgatan 13, 222 41 Lund, Sweden.

PMID: 32344523 PMCID: PMC7236018 DOI: 10.3390/diagnostics10040251

Abstract

The aims of this proof-of-concept study were to develop a collecting method for unstimulated parotid saliva in juvenile idiopathic arthritis (JIA) patients and healthy children and to investigate if inflammatory biomarkers could be detected in these samples. Forty-five children with JIA (median age of 12 years and 25th-75th percentile of 10-15 years; 33 girls and 12 boys) and 16 healthy children as controls (median age of 13 years and 25-75th percentile of 10-13 years; 11 girls and 5 boys) were enrolled in this study. Unstimulated parotid saliva was collected with a modified Carlson-Crittenden collector. The salivary flow rate and salivary concentrations of total protein and inflammatory mediators were assessed. The Meso Scale Discovery electrochemiluminescence immunoassay was used for analyzing protein concentrations and the inflammatory biomarkers. Sufficient parotid saliva volumes to be analyzed could be collected with the collection device. JIA patients had a lower sampling saliva volume (

Keywords: biomarkers; children; juvenile idiopathic arthritis; parotid saliva; saliva collection

References

  1. Acta Odontol Scand. 2005 Oct;63(5):284-93 - PubMed
  2. Pediatr Rheumatol Online J. 2018 Apr 25;16(1):32 - PubMed
  3. Eur J Dent Educ. 2008 Feb;12 Suppl 1:22-9 - PubMed
  4. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001 May;91(5):541-5 - PubMed
  5. Clin Chim Acta. 2007 Aug;383(1-2):30-40 - PubMed
  6. Oral Dis. 2005 Jan;11(1):27-34 - PubMed
  7. Swed Dent J. 2000;24(3):83-92 - PubMed
  8. Diabetes Metab Syndr Obes. 2019 Jul 23;12:1239-1247 - PubMed
  9. Arch Oral Biol. 2012 Jan;57(1):1-9 - PubMed
  10. PLoS One. 2013 Apr 24;8(4):e61356 - PubMed
  11. Ther Clin Risk Manag. 2014 Dec 22;11:45-51 - PubMed
  12. Acta Odontol Scand. 1998 Dec;56(6):360-5 - PubMed
  13. Lancet. 2011 Jun 18;377(9783):2138-49 - PubMed
  14. Cytokine. 2018 Jul;107:113-117 - PubMed
  15. J Rheumatol. 2004 Feb;31(2):390-2 - PubMed
  16. PLoS One. 2014 Dec 05;9(12):e114430 - PubMed
  17. Proteomics. 2003 Jun;3(6):1003-15 - PubMed
  18. PLoS One. 2014 Jun 10;9(6):e98799 - PubMed
  19. Scand J Pain. 2019 Dec 18;20(1):125-138 - PubMed
  20. Lancet. 2007 Mar 3;369(9563):767-778 - PubMed
  21. Arthritis Care Res (Hoboken). 2020 Jan;72(1):78-87 - PubMed
  22. Br Dent J. 1992 Apr 25;172(8):305-12 - PubMed
  23. Pediatr Dent. 1979 Mar;1(1):16-20 - PubMed
  24. J Immunol Methods. 2011 Sep 30;372(1-2):71-7 - PubMed
  25. Front Public Health. 2017 Apr 20;5:83 - PubMed
  26. J Dent Res. 1962 Jul-Aug;41:778-83 - PubMed
  27. Swed Dent J. 1984;8(6):271-6 - PubMed
  28. J Prosthet Dent. 2001 Feb;85(2):162-9 - PubMed
  29. J Periodontal Res. 1983 Nov;18(6):559-69 - PubMed
  30. Arch Oral Biol. 1993 Sep;38(9):751-4 - PubMed

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