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Nagarajan MB, Tentori AM, Zhang WC, et al. Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays. Microsyst Nanoeng. 2020;6:51doi: 10.1038/s41378-020-0169-8.
Nagarajan, M. B., Tentori, A. M., Zhang, W. C., Slack, F. J., & Doyle, P. S. (2020). Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays. Microsystems & nanoengineering, 651. https://doi.org/10.1038/s41378-020-0169-8
Nagarajan, Maxwell B, et al. "Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays." Microsystems & nanoengineering vol. 6 (2020): 51. doi: https://doi.org/10.1038/s41378-020-0169-8
Nagarajan MB, Tentori AM, Zhang WC, Slack FJ, Doyle PS. Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays. Microsyst Nanoeng. 2020 May 09;6:51. doi: 10.1038/s41378-020-0169-8. eCollection 2020. PMID: 32419951; PMCID: PMC7211184.
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Microsyst Nanoeng. 2020 May 09;6:51. doi: 10.1038/s41378-020-0169-8. eCollection 2020.

Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays.

Microsystems & nanoengineering

Maxwell B Nagarajan, Augusto M Tentori, Wen Cai Zhang, Frank J Slack, Patrick S Doyle

Affiliations

  1. 1Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139 USA.
  2. 2HMS Initiative for RNA Medicine, Department of Pathology, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, MA 02215 USA.

PMID: 32419951 PMCID: PMC7211184 DOI: 10.1038/s41378-020-0169-8

Abstract

Spatially resolved gene expression patterns are emerging as a key component of medical studies, including companion diagnostics, but technologies for quantification and multiplexing are limited. We present a method to perform spatially resolved and multiplexed microRNA (miRNA) measurements from formalin-fixed, paraffin-embedded (FFPE) tissue. Using nanoliter well arrays to pixelate the tissue section and photopatterned hydrogels to quantify miRNA, we identified differentially expressed miRNAs in tumors from a genetically engineered mouse model for non-small cell lung cancer (K-ras

© The Author(s) 2020.

Keywords: Chemistry; Engineering

Conflict of interest statement

Conflict of interestThe authors have filed a patent application on the nanoliter well array system and method. F.J.S. discloses financial interests and SAB roles with Mira DX or MiRNA Therapeutics.

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